{"id":9,"date":"2007-01-22T15:17:14","date_gmt":"2007-01-22T06:17:14","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=9"},"modified":"2023-10-03T15:49:30","modified_gmt":"2023-10-03T06:49:30","slug":"dna%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b9%e8%a7%a3%e6%9e%90%e3%82%b5%e3%83%bc%e3%83%93%e3%82%b9%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=9","title":{"rendered":"DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306b\u3064\u3044\u3066"},"content":{"rendered":"

\u5229\u7528\u306b\u3064\u3044\u3066\u306f\u6307\u5c0e\u6559\u54e1\u306e\u627f\u8afe\u3092\u53d7\u3051\u3001Webform\u3067\u7533\u3057\u8fbc\u307f\u3092\u884c\u3044\u307e\u3059\u3002\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u304f\u3060\u3055\u3044\u3002<\/span><\/p>\n

English version is “here<\/a><\/strong>“.<\/p>\n

\u3000
\n\u3000
\n\u25cf \u30b5\u30fc\u30d3\u30b9\u6982\u8981<\/strong><\/p>\n

DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306f\u6708\u30fb\u6c34\u30fb\u6728<\/strong><\/span>\u306e\u90313\u56de\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002
\n\u30c7\u30fc\u30bf\u306e\u9001\u4fe1\u306f\u901a\u5e38\u89e3\u6790\u65e5\u306e\u7fcc\u65e5<\/u>(\u706b\u30fb\u6728\u30fb\u91d1<\/strong><\/span>)\u3067\u3059\u3002
\n(\u4f46\u3057\u3001\u696d\u52d9\u306e\u90fd\u5408\u306b\u3088\u308a\u7d50\u679c\u9001\u4fe1\u304c\u9045\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059)<\/p>\n

\u30fb<\/strong>ABI3500xl Genetic Analyzer<\/strong><\/a>(\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc24\u672c\u30bf\u30a4\u30d7)\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002
\n\u30fb<\/strong>\u7d042.3\u6642\u9593\u306e\u6cf3\u52d5\u3067\u7d04800\u5869\u57fa(\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u304c\u826f\u597d\u306e\u5834\u5408)\u8aad\u3080\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002
\n\u30fb<\/strong>\u6cf3\u52d5\u64cd\u4f5c\u306f\u3059\u3079\u3066\u65bd\u8a2d\u30b9\u30bf\u30c3\u30d5\u304c\u884c\u3044\u307e\u3059\u3002
\n\u30fb<\/strong>\u7533\u8acb\u306f Webform<\/strong><\/a> \u3092\u3054\u5229\u7528\u304f\u3060\u3055\u3044(\u5b66\u5185\u306e\u307f\u30a2\u30af\u30bb\u30b9\u53ef\u80fd)\u3002
\n\u30fb<\/strong>\u8981\u9818\u306b\u5f93\u3044\u5fc5\u8981\u4e8b\u9805\u3092\u8a18\u5165\u3057\u3001\u78ba\u8a8d\u30dc\u30bf\u30f3\u3092\u62bc\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u60c5\u5831\u3092\u78ba\u8a8d\u5f8c\u3001\u9001\u4fe1\u3092\u62bc\u3059\u3068\u7ba1\u7406\u5ba4\u306be-mail\u304c\u9001\u4fe1\u3055\u308c\u307e\u3059\u3002<\/li>\n

\u30fb<\/strong>\u89e3\u6790\u3092\u958b\u59cb\u3059\u308b\u4e88\u5b9a\u65e5\u6642\u3092\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002
\n\u30fb<\/strong>\u4e88\u5b9a\u65e5\u6642\u306b\u5408\u308f\u305b\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u304f\u3060\u3055\u3044\u3002
\n\u30fb<\/strong>\u89e3\u6790\u7d42\u4e86\u5f8c\u3001e-mail\u3067\u30c7\u30fc\u30bf\u3092\u5727\u7e2e\u5f8c\u6dfb\u4ed8\u3057\u3066\u9001\u4fe1\u3057\u307e\u3059\u3002<\/p>\n

\u89e3\u6790\u306e\u9806\u756a\u306f\u5148\u7740\u9806\u3068\u306a\u308a\u307e\u3059\u3002\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u6539\u3081\u3066e-mail\u306b\u3088\u308a\u9023\u7d61\u3092\u3057\u307e\u3059\u3002\u89e3\u6790\u65e5\u306e\u6307\u5b9a\u306f\u3067\u304d\u307e\u305b\u3093<\/u>\u3002<\/p>\n

\u7533\u8acb\u2192\u4e88\u5b9a\u65e5\u78ba\u8a8d\u2192\u63d0\u51fa<\/span><\/strong> \u306e\u6d41\u308c\u3092\u5b88\u3063\u3066\u304f\u3060\u3055\u3044\u3002
\n\u3000
\n\u3000
\n\u25cf DNA\u30b5\u30f3\u30d7\u30eb\u306e\u8abf\u88fd\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u30fb\u5404\u81ea\u306e\u5b9f\u9a13\u5ba4\u3067\u306e\u51e6\u7406<\/strong><\/p>\n

\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u306b\u3088\u308b\u7cbe\u88fd+\u8131\u30a4\u30aa\u30f3\u30db\u30eb\u30e0\u30a2\u30df\u30c9\u6eb6\u89e3\u306720\u03bcl\u4ee5\u4e0a<\/strong><\/span>\u306e\u6eb6\u89e3\u6eb6\u6db2<\/strong><\/span><\/u>\u304c\u3001BigDyeX Terminater\u306b\u3088\u308b\u7cbe\u88fd\u306720\u00b5l\u4ee5\u4e0a<\/strong><\/span>\u306e\u4e0a\u6f84\u307f\u6eb6\u6db2<\/strong><\/span><\/u>\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u306e\u91cf\u306f\u63a8\u5968\u3067\u3042\u308a\u3001\u6642\u9593\u306e\u7d4c\u904e\u3068\u3068\u3082\u306b\u84b8\u767a\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u4f59\u88d5\u3092\u6301\u3063\u305f\u5bb9\u91cf\u3067\u63d0\u51fa\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\uff0a2016\u5e743\u6708\u3000BigDyeX Terminater\u7cbe\u88fd\u306e\u4e0a\u6f84\u307f\u91cf\u3092\u5909\u66f4(40\u00b5l\u4ee5\u4e0a \u2192 20\u00b5l\u4ee5\u4e0a)<\/p>\n

\u307e\u305f\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf+\u30db\u30eb\u30e0\u30a2\u30df\u30c9\u6eb6\u89e3\u306b\u3088\u308b\u65b9\u6cd5\u306e\u5834\u5408\u3001\u6eb6\u89e3\u5f8c\u306f2\u65e5\u9593<\/strong>\u7a0b\u5ea6\u7d4c\u3064\u3068\u86cd\u5149\u5f37\u5ea6\u306e\u4f4e\u4e0b\u304c\u898b\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002BigDyeX Terminator\u7cbe\u88fd\u6cd5\u306b\u3088\u308b\u5834\u5408\u306f\u5ba4\u6e29\u30672\u65e5\u9593<\/strong>\u3001\u51b7\u8535\u306710\u65e5\u9593<\/strong>\u7a0b\u5ea6\u4fdd\u5b58\u53ef\u80fd\u3067\u3059\u3002\u3044\u305a\u308c\u306e\u5834\u5408\u3082\u30a2\u30eb\u30df\u30db\u30a4\u30eb\u7b49\u3067\u906e\u5149\u3057\u305f\u5bb9\u5668\u5185\u3067\u4fdd\u5b58\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30fb\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u3078\u6301\u53c2\u3059\u308b<\/strong><\/p>\n

1. \u30c1\u30e5\u30fc\u30d6\u306b\u30b5\u30f3\u30d7\u30eb\u540d\u3092\u66f8\u304d\u3001\u65bd\u8a2d\u3067\u7528\u610f\u3057\u3066\u3044\u308b\u5c02\u7528\u30b1\u30fc\u30b9\u306b\u30b5\u30f3\u30d7\u30eb\u30c1\u30e5\u30fc\u30d6\u3092\u79fb\u3057\u3001\u30ab\u30d0\u30fc\u3092\u3059\u308b\u3002<\/p>\n

\uff0a\u30c1\u30e5\u30fc\u30d6\u306e\u30cd\u30fc\u30df\u30f3\u30b0\u3067\u306f\u30df\u30b9\u3092\u672a\u7136\u306b\u9632\u3050\u305f\u3081\u3001\u4ed6\u306e\u4eba\u306e\u30b5\u30f3\u30d7\u30eb\u3068\u533a\u5225\u304c\u3064\u304f\u3088\u3046\u306b\u8a18\u5165\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u9023\u756a\u306e\u6570\u5b57\u306f\u672b\u5c3e\u306b\u4ed8\u3051\u3066\u304f\u3060\u3055\u3044<\/strong><\/span>\u3002
\n(EX. 1,2,3…\u306e\u307f\u306e\u30cd\u30fc\u30df\u30f3\u30b0\u306e\u5834\u5408\u3001\u4ed6\u306e\u4eba\u3068\u533a\u5225\u304c\u96e3\u3057\u3044)<\/p>\n

\"\"<\/p>\n

2. \u5c02\u7528\u30b1\u30fc\u30b9\u306e\u30ab\u30d0\u30fc\u306b\u65e5\u4ed8\u3001\u6c0f\u540d\u3001\u30b5\u30f3\u30d7\u30eb\u6570\u3092\u66f8\u3044\u305f\u4ed8\u7b8b\u3092\u8cbc\u308a\u3001\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u306b\u3042\u308b\u51b7\u8535\u5eab\u306e\u6240\u5b9a\u306e\u5834\u6240\u306b\u5165\u308c\u308b\u3002<\/p>\n

\"\"
\n\u3000
\n\u3000
\n\u25cf \u30c7\u30fc\u30bf\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u30fb\u3054\u304f\u7a00\u306b\u3067\u3059\u304c\u3001\u5869\u57fa\u914d\u5217\u89e3\u6790\u7d50\u679c\u3092\u9001\u4ed8\u3057\u305f\u5f8c\u306b\u3001\u5229\u7528\u8005\u304b\u3089\u300cseq\u30d5\u30a1\u30a4\u30eb\u304c\u898b\u5f53\u305f\u3089\u306a\u3044\u306e\u3067\u518d\u5ea6\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044\u300d\u3068\u306e\u554f\u3044\u5408\u308f\u305b\u30e1\u30fc\u30eb\u3092\u9802\u304d\u307e\u3059\u3002
\n\u30fb\u901a\u5e38\u3001\u9001\u3089\u308c\u305f\u7d50\u679c\u306e\u4e2d\u306bseq\u30d5\u30a1\u30a4\u30eb\u304c\u7121\u3044\u5834\u5408\u306f\u3001\u89e3\u6790\u7d50\u679c\u304c\u4e0d\u826f\u3067\u3001\u914d\u5217\u3068\u3057\u3066\u6271\u3046\u3053\u3068\u304c\u3067\u304d\u306a\u304b\u3063\u305f\u5834\u5408\u304c\u307b\u3068\u3093\u3069\u3067\u3059\u3002\u305d\u306e\u5834\u5408\u306f\u3001\u9069\u5207\u306a\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3057\u3066\u30af\u30ed\u30de\u30c8(\u6ce2\u5f62)\u306e\u30d5\u30a1\u30a4\u30eb\u3092\u8868\u793a\u3059\u308b\u3068\u3001\u539f\u56e0\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u4e00\u52a9\u3068\u306a\u308b\u304b\u3068\u601d\u308f\u308c\u307e\u3059\u3002<\/p>\n

\u30fb\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u306eHP\u306b\u3001\u300c13 \u5b9f\u9a13\u30fb\u5229\u7528\u6cd5\u300d\u3068\u3044\u3046\u30da\u30fc\u30b8\u304c\u3042\u308a\u3001\u305d\u306e\u4e2d\u306b\u300cDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u6ce2\u5f62\u30c7\u30fc\u30bf\u7de8\u96c6\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3064\u3044\u3066<\/strong><\/a>\u300d\u3068\u3044\u3046\u8a18\u4e8b\u304c\u3042\u308a\u307e\u3059\u3002
\n\u30fbMAC OS\u3001Windows\u306b\u5bfe\u5fdc\u3057\u305f\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u60c5\u5831\u3092\u8f09\u305b\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u81ea\u8eab\u306ePC\u74b0\u5883\u306b\u3042\u3063\u305f\u3082\u306e\u3092\u3054\u4f7f\u7528\u304f\u3060\u3055\u3044\u3002
\n\u30fbWindows\u3067\u3042\u308c\u3070Lifetechnologies\u793e\u306eSequence Scanner Software\u304c\u4f7f\u3044\u3084\u3059\u3044\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3059\u308c\u3070ab1\u30d5\u30a1\u30a4\u30eb\u3088\u308a\u6ce2\u5f62\u30c7\u30fc\u30bf\u3092\u53c2\u7167\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059(Windows XP\u4ee5\u5916\u306b\u3082Windows7\u3068Windows10\u3067\u52d5\u4f5c\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059)\u3002
\n\u30fbMAC\u3067\u3082Sequence Scanner Software\u3092\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u8a73\u7d30\u306f\u300cMac\u5411\u3051Sequence Scanner\/Peak Scanner \u5c0e\u5165\u30de\u30cb\u30e5\u30a2\u30eb2_PlayOnMac_kagoshima-u ver.<\/strong><\/a>\u300d\u3092\u53c2\u7167\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb\u30af\u30ed\u30de\u30c8\u30c7\u30fc\u30bf\u306b\u3064\u3044\u3066\u3054\u4e0d\u660e\u306a\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u65bd\u8a2d\u307e\u3067\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002
\n\u3000
\n\u3000
\n\u25cf DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u6599\u91d1\u7b49\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u89e3\u6790\u6599\u91d1\uff1a\u300c\u3053\u3061\u3089\u300d<\/strong><\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u6d88\u8017\u54c1\u5206\u8b72\u4fa1\u683c\uff1a\u300c\u3053\u3061\u3089\u300d<\/strong><\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u3000\u25cb<\/strong>\u3000\u30b7\u30fc\u30af\u30a8\u30f3\u30b7\u30f3\u30b0\u30ad\u30c3\u30c8 BigDye Terminator v3.1(Life technologies)
\n\u3000\u3000\u30fb<\/strong>\u00d75 Sequence buffer(75\u00b5l)\u304c1\u672c\u4ed8\u3044\u3066\u304d\u307e\u3059(2\u672c\u76ee\u304b\u3089\u6709\u511f)\u3002
\n\u3000\u25cb<\/strong>\u3000Hi-Di Formamide(500\u03bcL\uff09
\n\u3000\u25cb<\/strong>\u3000\u7cbe\u88fd\u30ad\u30c3\u30c8 BigDyeX Terminator(Life technologies)<\/p>\n

\u4fa1\u683c\u306f\u3044\u305a\u308c\u3082\u5b66\u5185\u5229\u7528\u8005\u306e\u5834\u5408\u3067\u3059\u3002\u8a73\u7d30\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002
\n\u3000
\n\u25cf DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u95a2\u9023\u60c5\u5831<\/strong><\/p>\n

DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u95a2\u3059\u308b\u6709\u76ca\u306a\u8cc7\u6599\u3084\u60c5\u5831\u3092\u30db\u30fc\u30e0\u30da\u30fc\u30b8\u3067\u516c\u958b\u3057\u3066\u3044\u307e\u3059\u3002\u3054\u6d3b\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u3000\u300c\u3053\u3061\u3089\u300d<\/a><\/strong>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u5229\u7528\u306b\u3064\u3044\u3066\u306f\u6307\u5c0e\u6559\u54e1\u306e\u627f\u8afe\u3092\u53d7\u3051\u3001Webform\u3067\u7533\u3057\u8fbc\u307f\u3092\u884c\u3044\u307e\u3059\u3002\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u304f\u3060\u3055\u3044\u3002 English version is “here“. \u3000 \u3000 \u25cf \u30b5\u30fc\u30d3\u30b9\u6982\u8981 DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306f\u6708\u30fb\u6c34\u30fb\u6728\u306e\u90313\u56de\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002 \u30c7\u30fc\u30bf\u306e\u9001\u4fe1\u306f\u901a\u5e38\u89e3\u6790\u65e5\u306e\u7fcc\u65e5(\u706b\u30fb\u6728\u30fb\u91d1)\u3067\u3059\u3002 (\u4f46\u3057\u3001\u696d\u52d9\u306e\u90fd\u5408\u306b\u3088\u308a\u7d50\u679c\u9001\u4fe1\u304c\u9045\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059) \u30fbABI3500xl Genetic Analyzer(\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc24\u672c\u30bf\u30a4\u30d7)\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002 \u30fb\u7d042.3\u6642\u9593\u306e\u6cf3\u52d5\u3067\u7d04800\u5869\u57fa(\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u304c\u826f\u597d\u306e\u5834\u5408)\u8aad\u3080\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u30fb\u6cf3\u52d5\u64cd\u4f5c\u306f\u3059\u3079\u3066\u65bd\u8a2d\u30b9\u30bf\u30c3\u30d5\u304c\u884c\u3044\u307e\u3059\u3002 \u30fb\u7533\u8acb\u306f Webform \u3092\u3054\u5229\u7528\u304f\u3060\u3055\u3044(\u5b66\u5185\u306e\u307f\u30a2\u30af\u30bb\u30b9\u53ef\u80fd)\u3002 \u30fb\u8981\u9818\u306b\u5f93\u3044\u5fc5\u8981\u4e8b\u9805\u3092\u8a18\u5165\u3057\u3001\u78ba\u8a8d\u30dc\u30bf\u30f3\u3092\u62bc\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u60c5\u5831\u3092\u78ba\u8a8d\u5f8c\u3001\u9001\u4fe1\u3092\u62bc\u3059\u3068\u7ba1\u7406\u5ba4\u306be-mail\u304c\u9001\u4fe1\u3055\u308c\u307e\u3059\u3002 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\u30fb\u5404\u81ea\u306e\u5b9f\u9a13\u5ba4\u3067\u306e\u51e6\u7406 \u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf\u306b\u3088\u308b\u7cbe\u88fd+\u8131\u30a4\u30aa\u30f3\u30db\u30eb\u30e0\u30a2\u30df\u30c9\u6eb6\u89e3\u306720\u03bcl\u4ee5\u4e0a\u306e\u6eb6\u89e3\u6eb6\u6db2\u304c\u3001BigDyeX Terminater\u306b\u3088\u308b\u7cbe\u88fd\u306720\u00b5l\u4ee5\u4e0a\u306e\u4e0a\u6f84\u307f\u6eb6\u6db2\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u306e\u91cf\u306f\u63a8\u5968\u3067\u3042\u308a\u3001\u6642\u9593\u306e\u7d4c\u904e\u3068\u3068\u3082\u306b\u84b8\u767a\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u4f59\u88d5\u3092\u6301\u3063\u305f\u5bb9\u91cf\u3067\u63d0\u51fa\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \uff0a2016\u5e743\u6708\u3000BigDyeX Terminater\u7cbe\u88fd\u306e\u4e0a\u6f84\u307f\u91cf\u3092\u5909\u66f4(40\u00b5l\u4ee5\u4e0a \u2192 20\u00b5l\u4ee5\u4e0a) \u307e\u305f\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u6c88\u6bbf+\u30db\u30eb\u30e0\u30a2\u30df\u30c9\u6eb6\u89e3\u306b\u3088\u308b\u65b9\u6cd5\u306e\u5834\u5408\u3001\u6eb6\u89e3\u5f8c\u306f2\u65e5\u9593\u7a0b\u5ea6\u7d4c\u3064\u3068\u86cd\u5149\u5f37\u5ea6\u306e\u4f4e\u4e0b\u304c\u898b\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002BigDyeX Terminator\u7cbe\u88fd\u6cd5\u306b\u3088\u308b\u5834\u5408\u306f\u5ba4\u6e29\u30672\u65e5\u9593\u3001\u51b7\u8535\u306710\u65e5\u9593\u7a0b\u5ea6\u4fdd\u5b58\u53ef\u80fd\u3067\u3059\u3002\u3044\u305a\u308c\u306e\u5834\u5408\u3082\u30a2\u30eb\u30df\u30db\u30a4\u30eb\u7b49\u3067\u906e\u5149\u3057\u305f\u5bb9\u5668\u5185\u3067\u4fdd\u5b58\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u30fb\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u3078\u6301\u53c2\u3059\u308b 1. \u30c1\u30e5\u30fc\u30d6\u306b\u30b5\u30f3\u30d7\u30eb\u540d\u3092\u66f8\u304d\u3001\u65bd\u8a2d\u3067\u7528\u610f\u3057\u3066\u3044\u308b\u5c02\u7528\u30b1\u30fc\u30b9\u306b\u30b5\u30f3\u30d7\u30eb\u30c1\u30e5\u30fc\u30d6\u3092\u79fb\u3057\u3001\u30ab\u30d0\u30fc\u3092\u3059\u308b\u3002 \uff0a\u30c1\u30e5\u30fc\u30d6\u306e\u30cd\u30fc\u30df\u30f3\u30b0\u3067\u306f\u30df\u30b9\u3092\u672a\u7136\u306b\u9632\u3050\u305f\u3081\u3001\u4ed6\u306e\u4eba\u306e\u30b5\u30f3\u30d7\u30eb\u3068\u533a\u5225\u304c\u3064\u304f\u3088\u3046\u306b\u8a18\u5165\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u307e\u305f\u3001\u9023\u756a\u306e\u6570\u5b57\u306f\u672b\u5c3e\u306b\u4ed8\u3051\u3066\u304f\u3060\u3055\u3044\u3002 (EX. 1,2,3…\u306e\u307f\u306e\u30cd\u30fc\u30df\u30f3\u30b0\u306e\u5834\u5408\u3001\u4ed6\u306e\u4eba\u3068\u533a\u5225\u304c\u96e3\u3057\u3044) 2. \u5c02\u7528\u30b1\u30fc\u30b9\u306e\u30ab\u30d0\u30fc\u306b\u65e5\u4ed8\u3001\u6c0f\u540d\u3001\u30b5\u30f3\u30d7\u30eb\u6570\u3092\u66f8\u3044\u305f\u4ed8\u7b8b\u3092\u8cbc\u308a\u3001\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u306b\u3042\u308b\u51b7\u8535\u5eab\u306e\u6240\u5b9a\u306e\u5834\u6240\u306b\u5165\u308c\u308b\u3002 \u3000 \u3000 \u25cf \u30c7\u30fc\u30bf\u306b\u3064\u3044\u3066 \u30fb\u3054\u304f\u7a00\u306b\u3067\u3059\u304c\u3001\u5869\u57fa\u914d\u5217\u89e3\u6790\u7d50\u679c\u3092\u9001\u4ed8\u3057\u305f\u5f8c\u306b\u3001\u5229\u7528\u8005\u304b\u3089\u300cseq\u30d5\u30a1\u30a4\u30eb\u304c\u898b\u5f53\u305f\u3089\u306a\u3044\u306e\u3067\u518d\u5ea6\u9001\u4ed8\u3057\u3066\u304f\u3060\u3055\u3044\u300d\u3068\u306e\u554f\u3044\u5408\u308f\u305b\u30e1\u30fc\u30eb\u3092\u9802\u304d\u307e\u3059\u3002 \u30fb\u901a\u5e38\u3001\u9001\u3089\u308c\u305f\u7d50\u679c\u306e\u4e2d\u306bseq\u30d5\u30a1\u30a4\u30eb\u304c\u7121\u3044\u5834\u5408\u306f\u3001\u89e3\u6790\u7d50\u679c\u304c\u4e0d\u826f\u3067\u3001\u914d\u5217\u3068\u3057\u3066\u6271\u3046\u3053\u3068\u304c\u3067\u304d\u306a\u304b\u3063\u305f\u5834\u5408\u304c\u307b\u3068\u3093\u3069\u3067\u3059\u3002\u305d\u306e\u5834\u5408\u306f\u3001\u9069\u5207\u306a\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3057\u3066\u30af\u30ed\u30de\u30c8(\u6ce2\u5f62)\u306e\u30d5\u30a1\u30a4\u30eb\u3092\u8868\u793a\u3059\u308b\u3068\u3001\u539f\u56e0\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u4e00\u52a9\u3068\u306a\u308b\u304b\u3068\u601d\u308f\u308c\u307e\u3059\u3002 \u30fb\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u306eHP\u306b\u3001\u300c13 [Continue]<\/a><\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-9","post","type-post","status-publish","format-standard","hentry","category-dna"],"_links":{"self":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/9","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=9"}],"version-history":[{"count":156,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/9\/revisions"}],"predecessor-version":[{"id":19230,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/9\/revisions\/19230"}],"wp:attachment":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=9"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=9"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=9"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}