{"id":8594,"date":"2016-06-06T14:23:37","date_gmt":"2016-06-06T05:23:37","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=8594"},"modified":"2016-06-06T14:23:37","modified_gmt":"2016-06-06T05:23:37","slug":"%e6%8a%80%e8%a1%93%e8%ac%9b%e7%bf%92%e4%bc%9a%ef%bc%88%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr%ef%bc%89-2","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=8594","title":{"rendered":"\u6280\u8853\u8b1b\u7fd2\u4f1a\uff08\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff09"},"content":{"rendered":"<p>\u30a2\u30b8\u30ec\u30f3\u30c8\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u793e \u5b9a\u91cfPCR\u306b\u95a2\u3059\u308b\u30bb\u30df\u30ca\u30fc\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u65e5\u6642\uff1a2016\u5e746\u670823\u65e5(\u6728\u66dc)<br \/>\n\u3000\u3000\u3000\uff3b\u7b2c1\u90e8\uff3d\u300012\u664250\u5206\u301c14\u664220\u5206<br \/>\n\u3000\u3000\u3000\uff3b\u7b2c2\u90e8\uff3d\u300014\u664230\u5206\u301c15\u664240\u5206<br \/>\n\u3000\u3000\u3000\uff3b\u7b2c3\u90e8\uff3d\u300015\u664250\u5206\u301c16\u664220\u5206<br \/>\n\u3000\u3000\u3000\uff0a\u5404\u90e8\u3001\u9014\u4e2d\u9000\u5e2d\u306a\u3089\u3073\u306b\u9014\u4e2d\u53c2\u52a0\u304c\u53ef\u80fd\u3067\u3059\u3002\n<\/li>\n<\/ul>\n<ul>\n<li>\u6f14\u984c\uff1a\u5b9a\u91cfPCR\u6cd5 \u52c9\u5f37\u4f1a!\u3000\u5b9f\u9a13\u7cfb\u306e\u4f5c\u308a\u65b9\u30fb\u30c7\u30fc\u30bf\u89e3\u91c8\u30fb\u8ad6\u6587\u63b2\u8f09\u307e\u3067<\/li>\n<li>\u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3000\u30bb\u30df\u30ca\u30fc\u5ba4\uff081F\uff09<\/li>\n<li>\u62c5\u5f53\uff1a\u30a2\u30b8\u30ec\u30f3\u30c8\u30fb\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3000\u30d0\u30a4\u30aa\u55b6\u696d\u90e8\u3000\u5c3e\u5d0e<\/li>\n<\/ul>\n<ul>\n<li>\u6982\u8981\uff1a<br \/>\n\u30a2\u30b8\u30ec\u30f3\u30c8\u30fb\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u304b\u3089\u5b9a\u91cfPCR\u6cd5\u306b\u3064\u3044\u3066\u306e\u5b66\u8853\u30bb\u30df\u30ca\u30fc\u3092\u958b\u50ac\u3057\u307e\u3059\u3002<br \/>\n\u3053\u308c\u304b\u3089\u5b9a\u91cfPCR\u6cd5\u3092\u4f7f\u3063\u3066\u907a\u4f1d\u5b50\u5b9a\u91cf\u3092\u884c\u304a\u3046\u3068\u8a08\u753b\u3055\u308c\u3066\u3044\u308b\u65b9\u3092\u5bfe\u8c61\u3068\u3057\u3066\u3001\u5b9a\u91cfPCR\u306e\u57fa\u790e\u7406\u8ad6\u3001\u5b9f\u9a13\u7cfb\u69cb\u7bc9\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u306a\u3069\u3092\uff3b\u7b2c1\u90e8\uff3d\u3001\u3059\u3067\u306b\u5b9a\u91cfPCR\u3092\u884c\u308f\u308c\u3066\u3044\u308b\u65b9\u3005\u3078\u306f\u3001MIQE\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3092\u5229\u7528\u3057\u3066\u3001\u6b63\u3057\u3044\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u65b9\u6cd5\u3001\u5b9f\u9a13\u4e0a\u306e\u30d4\u30c3\u30c8\u30db\u30fc\u30eb\u3001\u30c7\u30fc\u30bf\u306e\u8ad6\u6587\u63b2\u8f09\u307e\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u306a\u3069\uff3b\u7b2c2\u90e8\uff3d\u3001\u6700\u8fd1\u7279\u306b\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u6838\u9178\u306e\u54c1\u8cea\u306b\u3088\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u3078\u306e\u5f71\u97ff\u306a\u3069\uff3b\u7b2c3\u90e8\uff3d\u306e\u30bb\u30df\u30ca\u30fc\u3092\u958b\u50ac\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\u3002<\/p>\n<p>\u304a\u5fd9\u3057\u3044\u6642\u671f\u3068\u306f\u5b58\u3058\u307e\u3059\u304c\u3001\u5b9a\u91cfPCR\u6cd5(\u907a\u4f1d\u5b50\u5b9a\u91cf)\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u3005(\u4ed6\u793e\u306e\u30b7\u30b9\u30c6\u30e0\u3054\u5229\u7528\u306e\u65b9\u3082\u5927\u6b53\u8fce\u3067\u3059)\u306e\u591a\u6570\u306e\u3054\u53c2\u52a0\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002\n<\/li>\n<\/ul>\n<ul>\n<li>\u5185\u5bb9\uff1a<\/ul>\n<ul>\n\uff3b\u7b2c1\u90e8\uff3d\u3000\u5b9a\u91cfPCR\u6cd5\u306e\u57fa\u790e<br \/>\n\u3000- \u5b9f\u9a13\u7cfb\u69cb\u7bc9 &#8211;<br \/>\n\u5b9a\u91cfPCR\u6cd5\u306e\u57fa\u672c\u539f\u7406\u3001\u5b9f\u9a13\u7cfb\u69cb\u7bc9(SYBR or TaqMan probe\u3001\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u3068\u76f8\u5bfe\u5b9a\u91cf\u6cd5\u3001\u76f8\u5bfe\u5b9a\u91cf(\u22bf\u22bfCt)\u6cd5\u306e\u57fa\u672c\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3001cDNA\u5408\u6210\u3001\u30d0\u30a4\u30aa\u30a2\u30ca\u30e9\u30a4\u30b6\u30fc\u306b\u3088\u308bRNA\u306e\u54c1\u8cea\u7ba1\u7406\u3001\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u9078\u629e\u3000\u306a\u3069<\/p>\n<p>\uff3b\u7b2c2\u90e8\uff3d\u3000\u5b9a\u91cfPCR\u6cd5\u306e\u5b9f\u9a13\u7cfb\u69cb\u7bc9<br \/>\n\u3000- \u305d\u306e\u30c7\u30fc\u30bf\u306e\u8ad6\u6587\u63b2\u8f09\u307e\u3067(MIQE \u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306e\u5229\u7528) &#8211;<br \/>\n\u5b9a\u91cfPCR\u6cd5\u306e\u30c7\u30fc\u30bf\u306e\u89e3\u6790\u30fb\u89e3\u91c8\u3001Standard curve\u89e3\u6790\u306b\u3088\u308b\u5b9f\u9a13\u306e\u8a55\u4fa1\u3001\u518d\u73fe\u6027\u3001ROX\u306eReference dye\u3068\u3057\u3066\u306e\u5229\u7528\u3001NTC\u306e\u30b7\u30b0\u30ca\u30eb\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3001\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790(\u22bf\u22bfCt\u6cd5\u306e\u30d4\u30c3\u30c8\u30db\u30fc\u30eb)\u3001\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u554f\u984c\u70b9\u3001\u8ad6\u6587\u63b2\u8f09\u307e\u3067\u306b\u5fc5\u8981\u306a\u3082\u306e\u3000\u306a\u3069<\/p>\n<p>\uff3b\u7b2c3\u90e8\uff3d\u3000\u30c8\u30d4\u30c3\u30af\u30b9! \u907a\u4f1d\u5b50\u5b9a\u91cf<br \/>\n\u3000- NGS\u89e3\u6790\u306a\u3069\u306e\u7d50\u679c\u306b\u5927\u304d\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u6838\u9178\u54c1\u8cea\u7ba1\u7406 &#8211;<br \/>\n\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3084\u30b2\u30ce\u30e0\u89e3\u6790\u306b\u304a\u3044\u3066\u3001\u78ba\u5b9f\u306a\u30c7\u30fc\u30bf\u3092\u5f97\u308b\u305f\u3081\u306b\u3001\u307e\u305f\u7d50\u679c\u306e\u89e3\u91c8\u3092\u6b63\u3057\u304f\u884c\u3046\u305f\u3081\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3067\u3042\u308bDNA\u3084RNA\u3001\u8abf\u88fd\u3055\u308c\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u306e\u54c1\u8cea\u304c\u3068\u3066\u3082\u91cd\u8981\u3067\u3059\u3002\u3053\u306e\u30bb\u30c3\u30b7\u30e7\u30f3\u3067\u306f\u30b5\u30f3\u30d7\u30ebQC\u3092\u7c21\u5358\u304b\u3064\u8fc5\u901f\u306b\u884c\u3046\u3053\u3068\u304c\u3067\u304d\u308b\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e4200 TapeStation\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\n<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u30a2\u30b8\u30ec\u30f3\u30c8\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u793e \u5b9a\u91cfPCR\u306b\u95a2\u3059\u308b\u30bb\u30df\u30ca\u30fc\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002 \u65e5\u6642\uff1a2016\u5e746\u670823\u65e5(\u6728\u66dc) \u3000\u3000\u3000\uff3b\u7b2c1\u90e8\uff3d\u300012\u664250\u5206\u301c14\u664220\u5206 \u3000\u3000\u3000\uff3b\u7b2c2\u90e8\uff3d\u300014\u664230\u5206\u301c15\u664240\u5206 \u3000\u3000\u3000\uff3b\u7b2c3\u90e8\uff3d\u300015\u664250\u5206\u301c16\u664220\u5206 \u3000\u3000\u3000\uff0a\u5404\u90e8\u3001\u9014\u4e2d\u9000\u5e2d\u306a\u3089\u3073\u306b\u9014\u4e2d\u53c2\u52a0\u304c\u53ef\u80fd\u3067\u3059\u3002 \u6f14\u984c\uff1a\u5b9a\u91cfPCR\u6cd5 \u52c9\u5f37\u4f1a!\u3000\u5b9f\u9a13\u7cfb\u306e\u4f5c\u308a\u65b9\u30fb\u30c7\u30fc\u30bf\u89e3\u91c8\u30fb\u8ad6\u6587\u63b2\u8f09\u307e\u3067 \u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3000\u30bb\u30df\u30ca\u30fc\u5ba4\uff081F\uff09 \u62c5\u5f53\uff1a\u30a2\u30b8\u30ec\u30f3\u30c8\u30fb\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3000\u30d0\u30a4\u30aa\u55b6\u696d\u90e8\u3000\u5c3e\u5d0e \u6982\u8981\uff1a \u30a2\u30b8\u30ec\u30f3\u30c8\u30fb\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u304b\u3089\u5b9a\u91cfPCR\u6cd5\u306b\u3064\u3044\u3066\u306e\u5b66\u8853\u30bb\u30df\u30ca\u30fc\u3092\u958b\u50ac\u3057\u307e\u3059\u3002 \u3053\u308c\u304b\u3089\u5b9a\u91cfPCR\u6cd5\u3092\u4f7f\u3063\u3066\u907a\u4f1d\u5b50\u5b9a\u91cf\u3092\u884c\u304a\u3046\u3068\u8a08\u753b\u3055\u308c\u3066\u3044\u308b\u65b9\u3092\u5bfe\u8c61\u3068\u3057\u3066\u3001\u5b9a\u91cfPCR\u306e\u57fa\u790e\u7406\u8ad6\u3001\u5b9f\u9a13\u7cfb\u69cb\u7bc9\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u306a\u3069\u3092\uff3b\u7b2c1\u90e8\uff3d\u3001\u3059\u3067\u306b\u5b9a\u91cfPCR\u3092\u884c\u308f\u308c\u3066\u3044\u308b\u65b9\u3005\u3078\u306f\u3001MIQE\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u3092\u5229\u7528\u3057\u3066\u3001\u6b63\u3057\u3044\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u65b9\u6cd5\u3001\u5b9f\u9a13\u4e0a\u306e\u30d4\u30c3\u30c8\u30db\u30fc\u30eb\u3001\u30c7\u30fc\u30bf\u306e\u8ad6\u6587\u63b2\u8f09\u307e\u3067\u306e\u30d7\u30ed\u30bb\u30b9\u306a\u3069\uff3b\u7b2c2\u90e8\uff3d\u3001\u6700\u8fd1\u7279\u306b\u6ce8\u76ee\u3055\u308c\u3066\u3044\u308b\u6838\u9178\u306e\u54c1\u8cea\u306b\u3088\u308b\u907a\u4f1d\u5b50\u89e3\u6790\u3078\u306e\u5f71\u97ff\u306a\u3069\uff3b\u7b2c3\u90e8\uff3d\u306e\u30bb\u30df\u30ca\u30fc\u3092\u958b\u50ac\u3055\u305b\u3066\u3044\u305f\u3060\u304d\u307e\u3059\u3002 \u304a\u5fd9\u3057\u3044\u6642\u671f\u3068\u306f\u5b58\u3058\u307e\u3059\u304c\u3001\u5b9a\u91cfPCR\u6cd5(\u907a\u4f1d\u5b50\u5b9a\u91cf)\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u3005(\u4ed6\u793e\u306e\u30b7\u30b9\u30c6\u30e0\u3054\u5229\u7528\u306e\u65b9\u3082\u5927\u6b53\u8fce\u3067\u3059)\u306e\u591a\u6570\u306e\u3054\u53c2\u52a0\u3092\u304a\u5f85\u3061\u3057\u3066\u304a\u308a\u307e\u3059\u3002 \u5185\u5bb9\uff1a \uff3b\u7b2c1\u90e8\uff3d\u3000\u5b9a\u91cfPCR\u6cd5\u306e\u57fa\u790e \u3000- \u5b9f\u9a13\u7cfb\u69cb\u7bc9 &#8211; \u5b9a\u91cfPCR\u6cd5\u306e\u57fa\u672c\u539f\u7406\u3001\u5b9f\u9a13\u7cfb\u69cb\u7bc9(SYBR or TaqMan probe\u3001\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u3068\u76f8\u5bfe\u5b9a\u91cf\u6cd5\u3001\u76f8\u5bfe\u5b9a\u91cf(\u22bf\u22bfCt)\u6cd5\u306e\u57fa\u672c\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3001cDNA\u5408\u6210\u3001\u30d0\u30a4\u30aa\u30a2\u30ca\u30e9\u30a4\u30b6\u30fc\u306b\u3088\u308bRNA\u306e\u54c1\u8cea\u7ba1\u7406\u3001\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u9078\u629e\u3000\u306a\u3069 \uff3b\u7b2c2\u90e8\uff3d\u3000\u5b9a\u91cfPCR\u6cd5\u306e\u5b9f\u9a13\u7cfb\u69cb\u7bc9 \u3000- \u305d\u306e\u30c7\u30fc\u30bf\u306e\u8ad6\u6587\u63b2\u8f09\u307e\u3067(MIQE \u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306e\u5229\u7528) &#8211; \u5b9a\u91cfPCR\u6cd5\u306e\u30c7\u30fc\u30bf\u306e\u89e3\u6790\u30fb\u89e3\u91c8\u3001Standard curve\u89e3\u6790\u306b\u3088\u308b\u5b9f\u9a13\u306e\u8a55\u4fa1\u3001\u518d\u73fe\u6027\u3001ROX\u306eReference dye\u3068\u3057\u3066\u306e\u5229\u7528\u3001NTC\u306e\u30b7\u30b0\u30ca\u30eb\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3001\u30b3\u30f3\u30bf\u30df\u30cd\u30fc\u30b7\u30e7\u30f3\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790(\u22bf\u22bfCt\u6cd5\u306e\u30d4\u30c3\u30c8\u30db\u30fc\u30eb)\u3001\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u554f\u984c\u70b9\u3001\u8ad6\u6587\u63b2\u8f09\u307e\u3067\u306b\u5fc5\u8981\u306a\u3082\u306e\u3000\u306a\u3069 \uff3b\u7b2c3\u90e8\uff3d\u3000\u30c8\u30d4\u30c3\u30af\u30b9! \u907a\u4f1d\u5b50\u5b9a\u91cf \u3000- NGS\u89e3\u6790\u306a\u3069\u306e\u7d50\u679c\u306b\u5927\u304d\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u6838\u9178\u54c1\u8cea\u7ba1\u7406 &#8211; 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