{"id":64,"date":"2007-10-10T14:17:46","date_gmt":"2007-10-10T05:17:46","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=64"},"modified":"2007-10-10T14:17:46","modified_gmt":"2007-10-10T05:17:46","slug":"%e6%8a%80%e8%a1%93%e8%ac%9b%e7%bf%92%e4%bc%9a%e3%83%9e%e3%82%a4%e3%82%af%e3%83%ad%e3%82%a2%e3%83%ac%e3%82%a4%e3%81%ae%e9%96%8b%e5%82%ac","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=64","title":{"rendered":"\u6280\u8853\u8b1b\u7fd2\u4f1a(\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4)\u306e\u958b\u50ac"},"content":{"rendered":"<p>\u4e0b\u8a18\u306e\u8981\u9818\u3067\u6280\u8853\u8b1b\u7fd2\u4f1a\u3092\u958b\u50ac\u3057\u307e\u3059\u3002\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\u3002<br \/>\n<center>\u8a18\u3000<\/center>\u3000<\/p>\n<ul>\n<li>\u6f14\u984c\uff1a\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30bb\u30df\u30ca\u30fc\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u6700\u524d\u7dda\u300c\u907a\u4f1d\u5b50\u767a\u73fe\u304b\u3089\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u307e\u3067<\/li>\n<li>\u8b1b\u5e2b\uff1a<a href=\"http:\/\/www.home.agilent.com\/agilent\/home.jspx?cc=JP&#038;lc=jpn&#038;NEWCCLC=JPjpn&#038;cmpid=4555\">\u30a2\u30b8\u30ec\u30f3\u30c8\u3000\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3000(\u682a)<\/a><\/li>\n<li>\u65e5\u6642\uff1a\u5e73\u621019\u5e7410\u670825\u65e5 (\u6728\u66dc\u65e5)<\/li>\n<p>\u5348\u5f8c1\u664230\u5206\u301c3\u6642<\/p>\n<li>\u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u30001F\u3000\u30bb\u30df\u30ca\u30fc\u5ba4<\/li>\n<li>\u5185\u5bb9<\/li>\n<\/ul>\n<blockquote><p>1.\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u57fa\u790e\uff08\u7d0440\u5206)<br \/>\n\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3092\u4f8b\u306b\u7528\u3044\u3066\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u5b9f\u9a13\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\uff12\uff0e\u30de\u30eb\u30c1\u30aa\u30df\u30af\u30b9\u6642\u4ee3\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308b\u9ad8\u611f\u5ea6\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u3054\u7d39\u4ecb\u3000\uff08\u7d0460\u5206\uff09\u3000<\/p>\n<li>\u65b0\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1a\u3000\u30aa\u30df\u30af\u30b9\u7814\u7a76\u3092\u52a0\u901f\u3059\u308b\u65b0\u3057\u3044\u30c4\u30fc\u30eb<\/li>\n<p>1\u679a\u306e\u30b9\u30e9\u30a4\u30c9\u30b0\u30e9\u30b9\u4e0a\u306b244,000\u3082\u306e\u9ad8\u7cbe\u5ea6\u30d7\u30ed\u30fc\u30d6\u306e\u642d\u8f09\u304c\u53ef\u80fd\u306b\u306a\u308a\u3001\u3055\u3089\u306b\u3001\u7d0460\uff05\u3082\u306e\u30b3\u30b9\u30c8\u30c0\u30a6\u30f3\u3092\u5b9f\u73fe\u3057\u305f\u3053\u3068\u3067\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u7528\u3044\u305f\u7814\u7a76\u306f\u65b0\u305f\u306a\u5c40\u9762\u3092\u8fce\u3048\u3066\u3044\u307e\u3059\u3002\u30a2\u30b8\u30ec\u30f3\u30c8\u793e\u306e\u30b7\u30b9\u30c6\u30e0\u306a\u3089\u3001\u5171\u901a\u306e\u30cf\u30fc\u30c9\u30a6\u30a8\u30a2\u3067\u3001miRNA\u3092\u306f\u3058\u3081\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u3001CGH\u3001ChIP-on-Chip\u89e3\u6790\u304c\u3067\u304d\u308b\u306e\u3067\u3001\u4eca\u6700\u3082\u30db\u30c3\u30c8\u306a\u7d71\u5408\u89e3\u6790\u306b\u6700\u9069\u3067\u3059\u3002<\/p>\n<li>miRNA: miRNA\u30a2\u30ec\u30a4\u3067\u958b\u62d3\u3059\u308b\u65b0\u9818\u57df<\/li>\n<p>\u308f\u305a\u304b100ng\u306etotal RNA\u304b\u3089\u9a5a\u304f\u307b\u3069\u7c21\u5358\u306bmiRNA\u306e\u7db2\u7f85\u7684\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u306e\u89e3\u6790\u304c\u3067\u304d\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u3001\u6b21\u4e16\u4ee3\u30a2\u30ec\u30a4\u89e3\u6790\u306b\u6c42\u3081\u3089\u308c\u308bqPCR\u3068\u306e\u76f8\u95a2\u6027\u3082\u826f\u597d\u3067\u3059\u3002<\/p>\n<li>\u907a\u4f1d\u5b50\u767a\u73fe\u30a2\u30ec\u30a4:\u3000\u57cb\u3082\u308c\u305f\u30d1\u30b9\u30a6\u30a7\u30a4\u3092\u6398\u308a\u8d77\u3053\u3059<\/li>\n<p>\u9ad8\u611f\u5ea6\u3001\u9ad8\u518d\u73fe\u6027\u3092\u8ffd\u53ca\u3057\u7d9a\u3051\u3066\u304d\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u30a2\u30ec\u30a4\u53f2\u4e0a\u521d\u306e\u9ad8\u611f\u5ea6\u3092\u5b9f\u73fe\u3057\u3001\u4f4e\u767a\u73fe\u9818\u57df\u306e\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3092\u53ef\u80fd\u306b\u3057\u307e\u3057\u305f\u3002\u3044\u3088\u3044\u3088\u3053\u308c\u307e\u3067\u898b\u3048\u306a\u304b\u3063\u305f\u30d1\u30b9\u30a6\u30a7\u30a4\uff0f\u30aa\u30f3\u30c8\u30ed\u30b8\u30fc\u89e3\u6790\u306e\u5e55\u958b\u3051\u3067\u3059\u3002<\/p>\n<li>\u30a2\u30ec\u30a4CGH\uff1a\u3000\u5fae\u7d30\u306a\u30b2\u30ce\u30e0\u69cb\u9020\u7570\u5e38\u3092\u7a81\u304d\u6b62\u3081\u308b<\/li>\n<p>\u30ce\u30a4\u30ba\u306e\u4f4e\u6e1b\u306b\u3053\u3060\u308f\u3063\u305f\u30c0\u30a4\u30ec\u30af\u30c8\u30e9\u30d9\u30ea\u30f3\u30b0\u3068\u9ad8\u54c1\u8cea\u306a\u30d7\u30ed\u30fc\u30d6\u8a2d\u8a08\u6280\u8853\u306b\u3088\u308a\u3001\u67d3\u8272\u4f53\u306e\u30b3\u30d4\u30fc\u6570\u306e\u7570\u5e38\u3092\u304d\u308f\u3081\u3066\u6b63\u78ba\u306b\u691c\u51fa\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u30c7\u30fc\u30bf\u306e\u54c1\u8cea\u306f\u751f\u30c7\u30fc\u30bf\u3092\u898b\u305f\u3060\u3051\u3067\u5206\u304b\u308a\u307e\u3059\u3002<\/p>\n<li>miRNA-\u907a\u4f1d\u5b50\u767a\u73fe-CGH\u7d71\u5408\u89e3\u6790\u306e\u3054\u7d39\u4ecb<\/li>\n<p>miRNA\u306e\u767a\u73fe\u30d7\u30ed\u30d5\u30a1\u30a4\u30eb\u304c\u3001\u764c\u306e\u7d44\u7e54\u3084\u7d30\u80de\u306e\u5206\u5316\u5ea6\u306e\u5f71\u97ff\u3092\u660e\u78ba\u306b\u53cd\u6620\u3057\u3066\u3044\u308b\u5831\u544a\u3082\u51fa\u3066\u304d\u3066\u3044\u307e\u3059\u3002<\/p>\n<li>\u30ed\u30b1\u30fc\u30b7\u30e7\u30f3\u89e3\u6790\uff1a\u3000\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u89e3\u660e\u3078\u306e\u30a2\u30d7\u30ed\u30fc\u30c1<\/li>\n<p>MIT\u306e\u30db\u30ef\u30a4\u30c8\u30d8\u30c3\u30c9\u7814\u7a76\u6240\u304c\u8003\u6848\u3057\u305f\u9ad8\u7cbe\u5ea6\u306e\u30bf\u30a4\u30ea\u30f3\u30b0\u30a2\u30ec\u30a4\u89e3\u6790\u304c\u3001ES\u7d30\u80de\u306b\u95a2\u4e0e\u3059\u308b\u8ee2\u5199\u56e0\u5b50\u306e\u5168\u30b2\u30ce\u30e0\u30de\u30c3\u30d4\u30f3\u30b0\u3092\u5b9f\u73fe\u3057\u307e\u3057\u305f\u3002\u514d\u75ab\u6c88\u964d\u3084\uff24\uff2e\uff21\u30e1\u30c1\u30eb\u5316\u5206\u6790\u6cd5\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u3001\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u306a\u8ee2\u5199\u8abf\u7bc0\u6a5f\u69cb\u3092\u89e3\u6790\u3059\u308b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30e9\u30a4\u30f3\u30ca\u30c3\u30d7\u3092\u3054\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002<\/p><\/blockquote>\n","protected":false},"excerpt":{"rendered":"<p>\u4e0b\u8a18\u306e\u8981\u9818\u3067\u6280\u8853\u8b1b\u7fd2\u4f1a\u3092\u958b\u50ac\u3057\u307e\u3059\u3002\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\u3002 \u8a18\u3000\u3000 \u6f14\u984c\uff1a\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30bb\u30df\u30ca\u30fc\u30b2\u30ce\u30df\u30af\u30b9\u7814\u7a76\u6700\u524d\u7dda\u300c\u907a\u4f1d\u5b50\u767a\u73fe\u304b\u3089\u30a8\u30d4\u30b8\u30a7\u30cd\u30c6\u30a3\u30af\u30b9\u307e\u3067 \u8b1b\u5e2b\uff1a\u30a2\u30b8\u30ec\u30f3\u30c8\u3000\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u3000(\u682a) \u65e5\u6642\uff1a\u5e73\u621019\u5e7410\u670825\u65e5 (\u6728\u66dc\u65e5) \u5348\u5f8c1\u664230\u5206\u301c3\u6642 \u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u30001F\u3000\u30bb\u30df\u30ca\u30fc\u5ba4 \u5185\u5bb9 1.\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u57fa\u790e\uff08\u7d0440\u5206) \u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3092\u4f8b\u306b\u7528\u3044\u3066\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u5b9f\u9a13\u306e\u6982\u8981\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002 \uff12\uff0e\u30de\u30eb\u30c1\u30aa\u30df\u30af\u30b9\u6642\u4ee3\u3092\u30b5\u30dd\u30fc\u30c8\u3059\u308b\u9ad8\u611f\u5ea6\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u3054\u7d39\u4ecb\u3000\uff08\u7d0460\u5206\uff09\u3000 \u65b0\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\uff1a\u3000\u30aa\u30df\u30af\u30b9\u7814\u7a76\u3092\u52a0\u901f\u3059\u308b\u65b0\u3057\u3044\u30c4\u30fc\u30eb 1\u679a\u306e\u30b9\u30e9\u30a4\u30c9\u30b0\u30e9\u30b9\u4e0a\u306b244,000\u3082\u306e\u9ad8\u7cbe\u5ea6\u30d7\u30ed\u30fc\u30d6\u306e\u642d\u8f09\u304c\u53ef\u80fd\u306b\u306a\u308a\u3001\u3055\u3089\u306b\u3001\u7d0460\uff05\u3082\u306e\u30b3\u30b9\u30c8\u30c0\u30a6\u30f3\u3092\u5b9f\u73fe\u3057\u305f\u3053\u3068\u3067\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u7528\u3044\u305f\u7814\u7a76\u306f\u65b0\u305f\u306a\u5c40\u9762\u3092\u8fce\u3048\u3066\u3044\u307e\u3059\u3002\u30a2\u30b8\u30ec\u30f3\u30c8\u793e\u306e\u30b7\u30b9\u30c6\u30e0\u306a\u3089\u3001\u5171\u901a\u306e\u30cf\u30fc\u30c9\u30a6\u30a8\u30a2\u3067\u3001miRNA\u3092\u306f\u3058\u3081\u3001\u907a\u4f1d\u5b50\u767a\u73fe\u3001CGH\u3001ChIP-on-Chip\u89e3\u6790\u304c\u3067\u304d\u308b\u306e\u3067\u3001\u4eca\u6700\u3082\u30db\u30c3\u30c8\u306a\u7d71\u5408\u89e3\u6790\u306b\u6700\u9069\u3067\u3059\u3002 miRNA: miRNA\u30a2\u30ec\u30a4\u3067\u958b\u62d3\u3059\u308b\u65b0\u9818\u57df \u308f\u305a\u304b100ng\u306etotal RNA\u304b\u3089\u9a5a\u304f\u307b\u3069\u7c21\u5358\u306bmiRNA\u306e\u7db2\u7f85\u7684\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u306e\u89e3\u6790\u304c\u3067\u304d\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u3001\u6b21\u4e16\u4ee3\u30a2\u30ec\u30a4\u89e3\u6790\u306b\u6c42\u3081\u3089\u308c\u308bqPCR\u3068\u306e\u76f8\u95a2\u6027\u3082\u826f\u597d\u3067\u3059\u3002 \u907a\u4f1d\u5b50\u767a\u73fe\u30a2\u30ec\u30a4:\u3000\u57cb\u3082\u308c\u305f\u30d1\u30b9\u30a6\u30a7\u30a4\u3092\u6398\u308a\u8d77\u3053\u3059 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