{"id":5719,"date":"2013-10-31T12:36:38","date_gmt":"2013-10-31T03:36:38","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=5719"},"modified":"2013-11-01T09:01:23","modified_gmt":"2013-11-01T00:01:23","slug":"%e6%8a%80%e8%a1%93%e8%ac%9b%e7%bf%92%e4%bc%9a%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr-6","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=5719","title":{"rendered":"\u6280\u8853\u8b1b\u7fd2\u4f1a(\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR)"},"content":{"rendered":"<p>\u30bf\u30ab\u30e9\u30d0\u30a4\u30aa\u793e 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\/>\n\u3000\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u539f\u7406\u3001\u5b9f\u9a13\u7cfb\u306e\u69cb\u7bc9\u3001\u76f8\u5bfe\u5b9a\u91cf\u7d50\u679c\u3092\u7fd2\u5f97\u3057\u305f\u3044\u65b9\u3001\u3053\u308c\u304b\u3089DNA\u767a\u73fe\u89e3\u6790\u3084\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u89e3\u6790\u3092\u884c\u3046\u65b9\u306b\u57fa\u790e\u304b\u3089\u767a\u73fe\u307e\u3067\u7d041\u6642\u9593\u306b\u308f\u305f\u308a\u8aac\u660e\u3055\u305b\u3066\u6234\u304d\u307e\u3059\u3002\u521d\u5fc3\u8005\u306e\u65b9\u3084\u3082\u3061\u308d\u3093\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u3054\u8208\u5473\u306e\u3042\u308b\u65b9\u3005\u306f\u6c17\u8efd\u306b\u3054\u53c2\u52a0\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u25cf\u3000\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3000\uff5e\u57fa\u790e\u304b\u3089\u767a\u73fe\u307e\u3067\uff5e<br \/>\n\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u6cd5\u306e\u539f\u7406\u3084\u5229\u70b9\u3001\u89e3\u6790\u65b9\u6cd5\u5b9f\u9a13\u306e\u6ce8\u610f\u70b9\u306a\u3069\u306b\u3064\u3044\u3066\u9806\u3092\u8ffd\u3063\u3066\u3054\u8aac\u660e\u3057\u307e\u3059\u3002<br \/>\n\u30fbPCR\uff06qPCR\u306e\u539f\u7406\u3001\u92f3\u578bRNA\u306e\u8abf\u6574<br \/>\n\u30fbqPCR\u5b9f\u9a13\u7cfb\u69cb\u7bc9\u30dd\u30a4\u30f3\u30c8\uff08\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3001Assay\u306e\u8a55\u4fa1\u30dd\u30a4\u30f3\u30c8\uff09<br \/>\n\u30fb\u76f8\u5bfe\u5b9a\u91cf\u89e3\u6790\uff08Reference\u907a\u4f1d\u5b50\u306e\u9078\u629e\uff0c\u691c\u91cf\u7dda vs \u0394\u0394Ct\u6cd5\uff09<br \/>\n\u30fb\u691c\u51fa\u539f\u7406\uff08SYBRGreen vs TaqMan Probe\uff09<br \/>\n\u307e\u305f\u3001\u30bf\u30ab\u30e9\u30d0\u30a4\u30aa\u682a\u5f0f\u4f1a\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8a66\u85ac\u3084\u88c5\u7f6e\u306e\u7279\u8272\u306b\u3064\u3044\u3066\u3082\u8a73\u3057\u304f\u3054\u7d39\u4ecb\u81f4\u3057\u307e\u3059\u3002<br \/>\n\u30fbRNA\u62bd\u51fa\u8a66\u85ac\u3001\u9006\u8ee2\u5199\u8a66\u85ac\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u69cb\u7bc9\u3001SYBRGreen\u2160\u8a66\u85ac\u3001\u30d7\u30ed\u30fc\u30d6\u6cd5\u8a66\u85ac\u3001Thermal Cycler Dice Real Time System\u7b49<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u30bf\u30ab\u30e9\u30d0\u30a4\u30aa\u793e \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u95a2\u3059\u308b\u30bb\u30df\u30ca\u30fc\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002 \u65e5\u6642\uff1a2013\u5e7411\u670814\u65e5(\u6728\u66dc)\u300014\u664200\u5206\uff5e15\u664200\u5206 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