{"id":24605,"date":"1978-03-31T09:10:32","date_gmt":"1978-03-31T00:10:32","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=24605"},"modified":"2026-04-08T17:03:21","modified_gmt":"2026-04-08T08:03:21","slug":"dna%e3%82%b7%e3%83%bc%e3%82%b1%e3%83%b3%e3%82%b9%e5%8f%97%e8%a8%97%e8%a7%a3%e6%9e%90-%e3%82%b5%e3%83%b3%e3%83%97%e3%83%ab%e8%aa%bf%e8%a3%bd%e3%81%ae%e3%82%ac%e3%82%a4%e3%83%89%e3%83%a9%e3%82%a4","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=24605","title":{"rendered":"DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 -\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3-"},"content":{"rendered":"<div style=\"background-color: #00549f; color: white; padding: 2px; font-size: 14px; text-align: center;\">\n    &#8211; DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 \u5229\u7528\u6599\u91d1 &#8211;\n<\/div>\n<p>\u3000<br \/>\n<C><\/p>\n<li>\u5229\u7528\u6599\u91d1\u306b\u3064\u3044\u3066\u306f<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=22393\" rel=\"noopener noreferrer\" target=\"_blank\">\u300c\u3053\u3061\u3089\u300d<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/li>\n<li>\u3054\u4e0d\u660e\u306a\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u7ba1\u7406\u5ba4(\u5185\u7dda\uff1a3581)\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u306b\u306a\u308b\u304b\u3001<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=8\" rel=\"noopener noreferrer\" target=\"_blank\">\u300c\u554f\u3044\u5408\u308f\u305b\u30d5\u30a9\u30fc\u30e0\u300d<\/a>\u304b\u3089\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/li>\n<\/ul>\n<p>\u3000<\/p>\n<div style=\"background-color: #00549f; color: white; padding: 2px; font-size: 14px; text-align: center;\">\n    &#8211; \u7533\u8acb\u624b\u9806 &#8211;\n<\/div>\n<p>\u3000<\/p>\n<ol>\n<li><a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=24846\" rel=\"noopener noreferrer\" target=\"_blank\">\u300cDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 \u7533\u8acb\u30d5\u30a9\u30fc\u30e0\u300d<\/a>\u304b\u3089\u7533\u8acb\u3059\u308b\u3002<br \/>\n\u3000\uff0a\u30e1\u30fc\u30eb\u30a2\u30c9\u30ec\u30b9\u306e\u9593\u9055\u3044\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/li>\n<li><span style=\"color: #3333ff;\">\u7533\u8acb\u5185\u5bb9\u304c\u30e1\u30fc\u30eb\u3067\u5c4a\u304d\u307e\u3059\u306e\u3067\u3001\u5fc5\u305a\u5185\u5bb9\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044<\/span>\u3002\n<\/ol>\n<p>\u3000<\/p>\n<div style=\"background-color: #00549f; color: white; padding: 2px; font-size: 14px; text-align: center;\">\n    &#8211; \u63d0\u51fa\u65b9\u6cd5 &#8211;\n<\/div>\n<p>\u3000<\/p>\n<ul>\n<li><span style=\"color: red;\">\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA + \u30d7\u30e9\u30a4\u30de\u30fc(6.4pmol\/1\u7a2e\u985e) = 14\u03bcl<\/span>\u3068\u306a\u308b\u3088\u3046\u306b<br \/>\n\t8\u9023tube<!--\u307e\u305f\u306f96well plate-->\u306b\u5206\u6ce8\u3059\u308b\u3002<br \/>\n\t\uff0a\u63d0\u51fa\u3057\u305f\u6df7\u5408\u7269\u306e\u534a\u91cf(7\u00b5l)\u3092\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u4f7f\u7528(10\u00b5l\u53cd\u5fdc\u7cfb)<\/p>\n<p>\t\u3053\u3061\u3089\u306e\u300c\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u81ea\u52d5\u8a08\u7b97\u30b7\u30fc\u30c8\u300d\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u30b5\u30a4\u30ba\u3084\u7a2e\u985e\u3092\u9078\u629e\u3059\u308b\u3068\u5fc5\u8981\u91cf\u304c\u81ea\u52d5\u3067\u8a08\u7b97\u3067\u304d\u307e\u3059\u3002<br \/>\n\u3000\u3000<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/application\/templateDNA_auto-calculation-sheet.xlsx\" rel=\"noopener\" target=\"_blank\"><span style=\"color: #009933;\"> \u25b6 &#8220;\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9&#8221; <\/span><\/a><\/p>\n<p>\t<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/templateDNA_auto-calculation-sheet.png\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/templateDNA_auto-calculation-sheet.png\" alt=\"\" width=\"368\" height=\"201\" class=\"aligncenter size-full wp-image-24667\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/templateDNA_auto-calculation-sheet.png 559w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/templateDNA_auto-calculation-sheet-300x165.png 300w\" sizes=\"auto, (max-width: 368px) 100vw, 368px\" \/><\/a><\/li>\n<li>\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306b\u3064\u3044\u3066\u306f\u4ee5\u4e0b\u306e\u6ce8\u610f\u4e8b\u9805\u3092\u53c2\u7167\u3059\u308b\u3002<br \/>\n\u3000<br \/>\n\t\u3010\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u54c1\u8cea\u3011<br \/>\n\t\u30fbPCR\u7523\u7269\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308a\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3001\u30b7\u30f3\u30b0\u30eb\u30d0\u30f3\u30c9\u3067\u3042\u308b\u3053\u3068\u3092\u5fc5\u305a\u78ba\u8a8d\u3059\u308b\u3002<br \/>\n\t\u30fbPCR\u7523\u7269\u306f\u672a\u53cd\u5fdc\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3084dNTP\u7b49\u9664\u53bb\u306e\u305f\u3081\u3001\u5fc5\u305a\u7cbe\u88fd\u3092\u884c\u3046\u3002<br \/>\n\t\u30fb\u5bb9\u91cf\u306e\u8abf\u6574\u306b\u306f\u3001\u6ec5\u83cc\u6c34\u3092\u4f7f\u7528\u3059\u308b\u3002<\/p>\n<p>\t\u3010\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u4f7f\u7528\u91cf\u3011<br \/>\n\t<u>\u591a\u3059\u304e\u308b\u5834\u5408<\/u>\uff1a\u8aad\u307f\u59cb\u3081\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u5f37\u304f\u3001\u89e3\u8aad\u8ddd\u96e2\u304c\u77ed\u304f\u306a\u308b\u3002<br \/>\n\t\u6ce2\u5f62\u30d4\u30fc\u30af\u304c\u691c\u51fa\u9650\u754c\u3092\u632f\u308a\u5207\u308a\u3001\u6b63\u78ba\u306b\u5869\u57fa\u3092\u8aad\u307f\u53d6\u308c\u306a\u3044\u3002<br \/>\n\t<u>\u5c11\u306a\u3059\u304e\u308b\u5834\u5408<\/u>\uff1a\u30b7\u30b0\u30ca\u30eb\u304c\u5f31\u304f\u3001\u4fe1\u983c\u6027\u304c\u4f4e\u3044\u30c7\u30fc\u30bf\u306b\u306a\u308b\u3002<\/p>\n<p>\t\u25c7 \u63d0\u51fa\u3059\u308bTemplate\u304a\u3088\u3073Primer\u91cf\u306e\u76ee\u5b89(14\u00b5l\u4e2d)<br \/>\n\u3000<br \/>\n\t<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount.png\" target=\"_blank\"\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount.png\" alt=\"\" width=\"315\" height=\"241\" class=\"aligncenter size-full wp-image-24619\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount.png 1485w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount-300x229.png 300w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount-1024x783.png 1024w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/dna-seq_temp-primer-amount-768x588.png 768w\" sizes=\"auto, (max-width: 315px) 100vw, 315px\" \/><\/a><br \/>\n\u3000<br \/>\n\t\u3010\u5bb9\u5668\u306e\u7a2e\u985e\u3011\u3000plate \/ 8\u9023tube<br \/>\n\t\u30fb\u30b5\u30f3\u30d7\u30eb\u6570\u304c48\u4ee5\u4e0b\u306e\u5834\u5408 \u2192 PCR\u75288\u9023tube\u306e\u307f<br \/>\n\t\u30fb\u30b5\u30f3\u30d7\u30eb\u6570\u304c49\u4ee5\u4e0a\u306e\u5834\u5408 \u2192 PCR\u75288\u9023tube\u306e\u307f(2026.4\u73fe\u5728)<!--96well plate(PCR\u75288\u9023tube\u3067\u3082OK)--><br \/>\n\t\u30fb\u5bb9\u91cf\u306e\u8abf\u6574\u306b\u306f\u3001\u6ec5\u83cc\u6c34\u3092\u4f7f\u7528\u3059\u308b\u3002<\/p>\n<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-first \">\n<a href=\".\/wp-content\/uploads\/2026\/03\/8-striptube-1.jpg\" title=\"8-striptube-1\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2026\/03\/8-striptube-1.jpg\" title=\"8-striptube-1\" width=\"178\" height=\"48\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-24633\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-1.jpg 894w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-1-300x81.jpg 300w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-1-768x208.jpg 768w\" sizes=\"auto, (max-width: 178px) 100vw, 178px\" \/><\/a><br \/>\n<a href=\".\/wp-content\/uploads\/2026\/03\/8-striptube-2.png\" title=\"8-striptube-2\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2026\/03\/8-striptube-2.png\" title=\"8-striptube-2\" width=\"195\" height=\"113\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-24634\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-2.png 977w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-2-300x173.png 300w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/8-striptube-2-768x443.png 768w\" sizes=\"auto, (max-width: 195px) 100vw, 195px\" \/><\/a>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-last \">\n<span style=\"background-color: #ffff99;\">8\u9023tube<\/span>\uff1aPCR\u75288\u9023tube(<span style=\"color: #3333ff;\"><u>\u5bb9\u91cf 0.2ml<\/u><\/span>)<br \/>\n\t\uff0a\u6ce8\u610f\u3000\u84cb\u304c\u5225\u306e\u30bf\u30a4\u30d7<\/p>\n<p>\u30fb\u30c1\u30e5\u30fc\u30d6\u306b\u901a\u3057\u756a\u53f7\u3092\u3064\u3051\u308b<br \/>\n\u30fb<u>\u7533\u8acb\u8005\u306e\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8(3\u6587\u5b57)<\/u>\u3092\u8a18\u8f09\u3059\u308b\n<\/div>\n<\/div>\n<p><!--\n\u3000\n[wc_row]\n[wc_column size=\"one-half\" position=\"first\"]\n<a href=\".\/wp-content\/uploads\/2026\/03\/96well-plate-4.png\" title=\"96well-plate-4\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2026\/03\/96well-plate-4.png\" title=\"96well-plate-4\" width=\"206\" height=\"104\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-24641\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-4.png 573w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-4-300x152.png 300w\" sizes=\"auto, (max-width: 206px) 100vw, 206px\" \/><\/a>\n[\/wc_column]\n[wc_column size=\"one-half\" position=\"last\"]\n<span style=\"background-color: #ffff99;\">96well plate<\/span>\uff1aApplied Biosystems 3500\/3500XL\/3130XL\u306b\u9069\u5408\u3057\u305f<span style=\"color: #3333ff;\"><u>\u5bb9\u91cf 0.2ml<\/u><\/span>\n\t\u4f8b\uff09\u30a4\u30ca\u30fb\u30aa\u30d7\u30c6\u30a3\u30ab\uff1a3540-00\n\t\u3000\u3000ExtraGene\uff1aP-96-PF\n[\/wc_column]\n[\/wc_row]\n\u3000\n\t\u301096well plate\u3067\u306e\u914d\u7f6e\u3011\u3000\u30b5\u30f3\u30d7\u30eb\u306e\u6ce8\u5165\u4f8b(50\u30b5\u30f3\u30d7\u30eb\u306e\u5834\u5408)\n\n<a href=\".\/wp-content\/uploads\/2026\/03\/96well-plate-2.png\" title=\"96well-plate-2\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2026\/03\/96well-plate-2.png\" title=\"96well-plate-2\" width=\"397\" height=\"165\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-24656\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-2.png 1772w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-2-300x125.png 300w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-2-1024x426.png 1024w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-2-768x320.png 768w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2026\/03\/96well-plate-2-1536x640.png 1536w\" sizes=\"auto, (max-width: 397px) 100vw, 397px\" \/><\/a>\n\n\t\u3000\u3000\u3000\u3000\u3000\u3000\u2605 A1,B1,C1\u2026\u306e\u9806\u3067\u3001\u7e26\u306b8\u30b5\u30f3\u30d7\u30eb\u305a\u3064\u5165\u308c\u308b\n\t\u3000\u3000\u3000\u3000\u3000\u3000\u2605 \u9593\u306f\u7a7a\u3051\u305a\u3001\u5de6\u4e0a\u304b\u3089\u8a70\u3081\u3066\u6ce8\u5165\u3059\u308b\n<\/ul>\n\n\n--><\/p>\n","protected":false},"excerpt":{"rendered":"<p>&#8211; DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 \u5229\u7528\u6599\u91d1 &#8211; \u3000 \u5229\u7528\u6599\u91d1\u306b\u3064\u3044\u3066\u306f\u300c\u3053\u3061\u3089\u300d\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002 \u3054\u4e0d\u660e\u306a\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u7ba1\u7406\u5ba4(\u5185\u7dda\uff1a3581)\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u306b\u306a\u308b\u304b\u3001\u300c\u554f\u3044\u5408\u308f\u305b\u30d5\u30a9\u30fc\u30e0\u300d\u304b\u3089\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002 \u3000 &#8211; \u7533\u8acb\u624b\u9806 &#8211; \u3000 \u300cDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 \u7533\u8acb\u30d5\u30a9\u30fc\u30e0\u300d\u304b\u3089\u7533\u8acb\u3059\u308b\u3002 \u3000\uff0a\u30e1\u30fc\u30eb\u30a2\u30c9\u30ec\u30b9\u306e\u9593\u9055\u3044\u306b\u6ce8\u610f\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u7533\u8acb\u5185\u5bb9\u304c\u30e1\u30fc\u30eb\u3067\u5c4a\u304d\u307e\u3059\u306e\u3067\u3001\u5fc5\u305a\u5185\u5bb9\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u3000 &#8211; \u63d0\u51fa\u65b9\u6cd5 &#8211; \u3000 \u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA + \u30d7\u30e9\u30a4\u30de\u30fc(6.4pmol\/1\u7a2e\u985e) = 14\u03bcl\u3068\u306a\u308b\u3088\u3046\u306b 8\u9023tube\u306b\u5206\u6ce8\u3059\u308b\u3002 \uff0a\u63d0\u51fa\u3057\u305f\u6df7\u5408\u7269\u306e\u534a\u91cf(7\u00b5l)\u3092\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u4f7f\u7528(10\u00b5l\u53cd\u5fdc\u7cfb) \u3053\u3061\u3089\u306e\u300c\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u81ea\u52d5\u8a08\u7b97\u30b7\u30fc\u30c8\u300d\u3092\u4f7f\u7528\u3059\u308b\u3068\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u30b5\u30a4\u30ba\u3084\u7a2e\u985e\u3092\u9078\u629e\u3059\u308b\u3068\u5fc5\u8981\u91cf\u304c\u81ea\u52d5\u3067\u8a08\u7b97\u3067\u304d\u307e\u3059\u3002 \u3000\u3000 \u25b6 &#8220;\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9&#8221; \u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306b\u3064\u3044\u3066\u306f\u4ee5\u4e0b\u306e\u6ce8\u610f\u4e8b\u9805\u3092\u53c2\u7167\u3059\u308b\u3002 \u3000 \u3010\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u54c1\u8cea\u3011 \u30fbPCR\u7523\u7269\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306b\u3088\u308a\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3001\u30b7\u30f3\u30b0\u30eb\u30d0\u30f3\u30c9\u3067\u3042\u308b\u3053\u3068\u3092\u5fc5\u305a\u78ba\u8a8d\u3059\u308b\u3002 \u30fbPCR\u7523\u7269\u306f\u672a\u53cd\u5fdc\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u3084dNTP\u7b49\u9664\u53bb\u306e\u305f\u3081\u3001\u5fc5\u305a\u7cbe\u88fd\u3092\u884c\u3046\u3002 \u30fb\u5bb9\u91cf\u306e\u8abf\u6574\u306b\u306f\u3001\u6ec5\u83cc\u6c34\u3092\u4f7f\u7528\u3059\u308b\u3002 \u3010\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u4f7f\u7528\u91cf\u3011 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