{"id":242,"date":"2009-02-03T09:44:51","date_gmt":"2009-02-03T00:44:51","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=242"},"modified":"2009-02-03T09:44:51","modified_gmt":"2009-02-03T00:44:51","slug":"bigdye-xterminator-%e7%b2%be%e8%a3%bd%e3%82%ad%e3%83%83%e3%83%88%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6-2","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=242","title":{"rendered":"BigDye XTerminator \u7cbe\u88fd\u30ad\u30c3\u30c8\u306b\u3064\u3044\u3066"},"content":{"rendered":"

BigDyeX\u3092\u5229\u7528\u3055\u308c\u3066\u3046\u307e\u304f\u3044\u3063\u305f\u308a\u3001\u5168\u304f\u3046\u307e\u304f\u3044\u304b\u306a\u304b\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002
\n\u4ee5\u4e0b\u306f\u65bd\u8a2dML genet\u3067\u6d41\u308c\u305f\u60c5\u5831\u3067\u3059\u3002\u53c2\u8003\u306b\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

    \n
  1. \n

    \u4e00\u3064\u306f
    \n\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306e\u5f37\u3055\u304c\u4e0d\u8db3\u3057\u3066\u3044\u308b\u3053\u3068\u3067\u3059\u3002
    \n\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3067\u306fTUPLEMIXER TWIN3-28N(IWAKI)\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u4e8b\u5b9f\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306e\u5f37\u3055\u304c\u4e0d\u8db3\u3057\u3066\u3044\u305f\u305f\u3081\u306b\u8aad\u3081\u306a\u304b\u3063\u305f\u3068\u3053\u308d\u304c\u3042\u308a\u3001\u5f53\u65bd\u8a2d\u306e\u3082\u306e\u3092\u4f7f\u7528\u3059\u308b\u3068\u8aad\u3081\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u4e8b\u4f8b\u304c\u3042\u308a\u307e\u3059\u3002\u5f53\u65bd\u8a2d\u306e\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3092\u8a66\u9a13\u7684\u306b\u4f7f\u3044\u305f\u3044\u65b9\u306f3F RNA\u5b9f\u9a13\u5ba4\u306b\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u5229\u7528\u4e0b\u3055\u3044\u3002<\/p>\n

    \u3082\u3046\u4e00\u3064\u304c
    \n\u300c\u901a\u5e38\u306e\u30c1\u30c3\u30d7\u3092\u4f7f\u7528\u3059\u308b\u3068\u5f84\u304c\u7d30\u3059\u304e\u308b\u305f\u3081\u30d3\u30fc\u30ba\u304c\u5341\u5206\u6458\u51fa\u3067\u304d\u306a\u3044\u300d
    \n\u3068\u3044\u3046\u53ef\u80fd\u6027\u3067\u3059\u3002
    \n\u65bd\u8a2d\u3067\u3082BigDyeX\u3092\u4f7f\u7528\u3057\u3066\u89e3\u6790\u3059\u308b\u306e\u3067\u3059\u304c\u3001\u86cd\u5149\u5f37\u5ea6\u304c1,000\u30aa\u30fc\u30d0\u30fc\u3059\u308b\u3053\u3068\u3082\u3042\u308c\u3070\u3001150\u7a0b\u5ea6\u306b\u306a\u308b\u3053\u3068\u304c\u591a\u3005\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u8981\u56e0\u306f\u3053\u308c\u3060\u3063\u305f\u306e\u304b\u3082\u77e5\u308c\u307e\u305b\u3093\u3002
    \n\u662f\u975e\u304a\u8a66\u3057\u4e0b\u3055\u3044\u3002<\/p>\n<\/blockquote>\n<\/li>\n

  2. \n

    \nX-terminator\u306b\u95a2\u3057\u307e\u3057\u30661\/4\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u884c\u306a\u3046\u969b\u306e\u6ce8\u610f\u70b9\u3092\u805e\u304d\u307e\u3057\u305f\u306e\u3067\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002<\/p>\n

    BigDye XTerminator \u7cbe\u88fd\u30ad\u30c3\u30c8\u306e\u5229\u7528\u65b9\u6cd5\u306f\u3001\u4ee5\u4e0b\u3092\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

    A
    \nBigDye Terminator3.1 1\/4\u91cf\u30685X Buffer\u3092\u7528\u3044\u3066\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e020uL\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u884c\u3046\u5834\u5408\u306f\u3001\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u901a\u308a\u306e\u4f7f\u7528\u91cf\u3067\u7cbe\u88fd\u3057\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u306e\u6dfb\u52a0\u91cf\u306f\u3001\u4ee5\u4e0b\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

    \u53cd\u5fdc\u6e08\u307f\u30b5\u30f3\u30d7\u30eb 20uL
    \nSAM\u6eb6\u6db2 90uL
    \nXterminator\u6eb6\u6db2 20uL<\/p>\n

    B
    \nBigDye Terminator3.1 1\/4\u91cf\u3067\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e0\u30821\/4\u306e5uL\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u884c\u3046\u5834\u5408\u306f\u3001BDX\u306e\u4f7f\u7528\u91cf\u30821\/4\u306b\u3057\u307e\u3059\u3002
    \n\u3053\u306e\u5834\u5408\u306e\u6dfb\u52a0\u91cf\u306f\u3001\u4ee5\u4e0b\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n

    \u53cd\u5fdc\u6e08\u307f\u30b5\u30f3\u30d7\u30eb 5uL
    \nSAM\u6eb6\u6db2 22.5uL
    \nXterminator\u6eb6\u6db2 5uL<\/p>\n

    3130\u7b49BDX\u5c02\u7528\u30e2\u30b8\u30e5\u30fc\u30eb\u3092\u4f7f\u3044\u30e9\u30f3\u30cb\u30f3\u30b0\u3059\u308b\u5834\u5408\u306b\u3001B\u306e\u6761\u4ef6\u3067\u7cbe\u88fd\u3059\u308b\u3068\u6eb6\u6db2\u91cf\u304c\u5c11\u306a\u3044\u305f\u3081\u30b5\u30f3\u30d7\u30eb\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u884c\u308f\u308c\u306a\u304f\u306a\u308a\u307e\u3059\u3002
    \n\u3053\u306e\u5834\u5408\u306f\u30015X Buffer\u3092\u7528\u3044\u3066\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e020uL\u3067\u53cd\u5fdc\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3044\u305f\u3057\u307e\u3059\u3002\uff08A\u306e\u6761\u4ef6\uff09
    \n\u53cd\u5fdc\u7cfb\u306e\u5909\u66f4\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u3001\u53cd\u5fdc\u5f8c\u30b5\u30f3\u30d7\u30eb\u306b\u6c3415uL\u3092\u52a0\u3048A\u306e\u6761\u4ef6\u3067\u7cbe\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

    \u30dd\u30a4\u30f3\u30c8\uff1a
    \nBigDye Terminator3.1 1\/4\u91cf\u3067\u53cd\u5fdc\u3059\u308b\u5834\u5408\u306f\u3001\u652a\u62cc\u6642\u9593\u3092\u77ed\u304f\u3057\u305f\u307b\u3046\u304c\u826f\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002
    \n10-20\u5206\u7a0b\u5ea6\u306e\u77ed\u3044\u652a\u62cc\u6642\u9593\u3067\u3082\u30c6\u30b9\u30c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

    \u91cd\u8981\uff1a
    \n\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u30df\u30ad\u30b5\u30fc\u3067\u652a\u62cc\u3059\u308b\u6642\u306f\u300196well Plate\u3082\u3057\u304f\u306f0.2mL\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u3044\u307e\u3059\u3002
    \n1.5mL\u7b49\u306e\u30c1\u30e5\u30fc\u30d6\u306a\u3069\u3067\u306f\u652a\u62cc\u52b9\u7387\u304c\u5909\u308f\u308b\u305f\u3081\u554f\u984c\u304c\u8d77\u3053\u308a\u307e\u3059\u3002\uff08\u7cbe\u88fd\u4e0d\u826f\u3084\u30b7\u30b0\u30ca\u30eb\u4f4e\u4e0b\uff09<\/p><\/blockquote>\n<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    BigDyeX\u3092\u5229\u7528\u3055\u308c\u3066\u3046\u307e\u304f\u3044\u3063\u305f\u308a\u3001\u5168\u304f\u3046\u307e\u304f\u3044\u304b\u306a\u304b\u3063\u305f\u308a\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002 \u4ee5\u4e0b\u306f\u65bd\u8a2dML genet\u3067\u6d41\u308c\u305f\u60c5\u5831\u3067\u3059\u3002\u53c2\u8003\u306b\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002 \u4e00\u3064\u306f \u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306e\u5f37\u3055\u304c\u4e0d\u8db3\u3057\u3066\u3044\u308b\u3053\u3068\u3067\u3059\u3002 \u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3067\u306fTUPLEMIXER TWIN3-28N(IWAKI)\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u4e8b\u5b9f\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306e\u5f37\u3055\u304c\u4e0d\u8db3\u3057\u3066\u3044\u305f\u305f\u3081\u306b\u8aad\u3081\u306a\u304b\u3063\u305f\u3068\u3053\u308d\u304c\u3042\u308a\u3001\u5f53\u65bd\u8a2d\u306e\u3082\u306e\u3092\u4f7f\u7528\u3059\u308b\u3068\u8aad\u3081\u308b\u3088\u3046\u306b\u306a\u3063\u305f\u4e8b\u4f8b\u304c\u3042\u308a\u307e\u3059\u3002\u5f53\u65bd\u8a2d\u306e\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u3092\u8a66\u9a13\u7684\u306b\u4f7f\u3044\u305f\u3044\u65b9\u306f3F RNA\u5b9f\u9a13\u5ba4\u306b\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u5229\u7528\u4e0b\u3055\u3044\u3002 \u3082\u3046\u4e00\u3064\u304c \u300c\u901a\u5e38\u306e\u30c1\u30c3\u30d7\u3092\u4f7f\u7528\u3059\u308b\u3068\u5f84\u304c\u7d30\u3059\u304e\u308b\u305f\u3081\u30d3\u30fc\u30ba\u304c\u5341\u5206\u6458\u51fa\u3067\u304d\u306a\u3044\u300d \u3068\u3044\u3046\u53ef\u80fd\u6027\u3067\u3059\u3002 \u65bd\u8a2d\u3067\u3082BigDyeX\u3092\u4f7f\u7528\u3057\u3066\u89e3\u6790\u3059\u308b\u306e\u3067\u3059\u304c\u3001\u86cd\u5149\u5f37\u5ea6\u304c1,000\u30aa\u30fc\u30d0\u30fc\u3059\u308b\u3053\u3068\u3082\u3042\u308c\u3070\u3001150\u7a0b\u5ea6\u306b\u306a\u308b\u3053\u3068\u304c\u591a\u3005\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u8981\u56e0\u306f\u3053\u308c\u3060\u3063\u305f\u306e\u304b\u3082\u77e5\u308c\u307e\u305b\u3093\u3002 \u662f\u975e\u304a\u8a66\u3057\u4e0b\u3055\u3044\u3002 X-terminator\u306b\u95a2\u3057\u307e\u3057\u30661\/4\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u884c\u306a\u3046\u969b\u306e\u6ce8\u610f\u70b9\u3092\u805e\u304d\u307e\u3057\u305f\u306e\u3067\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002 BigDye XTerminator \u7cbe\u88fd\u30ad\u30c3\u30c8\u306e\u5229\u7528\u65b9\u6cd5\u306f\u3001\u4ee5\u4e0b\u3092\u53c2\u7167\u304f\u3060\u3055\u3044\u3002 A BigDye Terminator3.1 1\/4\u91cf\u30685X Buffer\u3092\u7528\u3044\u3066\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e020uL\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u884c\u3046\u5834\u5408\u306f\u3001\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u901a\u308a\u306e\u4f7f\u7528\u91cf\u3067\u7cbe\u88fd\u3057\u307e\u3059\u3002\u3053\u306e\u5834\u5408\u306e\u6dfb\u52a0\u91cf\u306f\u3001\u4ee5\u4e0b\u306b\u306a\u308a\u307e\u3059\u3002 \u53cd\u5fdc\u6e08\u307f\u30b5\u30f3\u30d7\u30eb 20uL SAM\u6eb6\u6db2 90uL Xterminator\u6eb6\u6db2 20uL B BigDye Terminator3.1 1\/4\u91cf\u3067\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e0\u30821\/4\u306e5uL\u3067\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u884c\u3046\u5834\u5408\u306f\u3001BDX\u306e\u4f7f\u7528\u91cf\u30821\/4\u306b\u3057\u307e\u3059\u3002 \u3053\u306e\u5834\u5408\u306e\u6dfb\u52a0\u91cf\u306f\u3001\u4ee5\u4e0b\u306b\u306a\u308a\u307e\u3059\u3002 \u53cd\u5fdc\u6e08\u307f\u30b5\u30f3\u30d7\u30eb 5uL SAM\u6eb6\u6db2 22.5uL Xterminator\u6eb6\u6db2 5uL 3130\u7b49BDX\u5c02\u7528\u30e2\u30b8\u30e5\u30fc\u30eb\u3092\u4f7f\u3044\u30e9\u30f3\u30cb\u30f3\u30b0\u3059\u308b\u5834\u5408\u306b\u3001B\u306e\u6761\u4ef6\u3067\u7cbe\u88fd\u3059\u308b\u3068\u6eb6\u6db2\u91cf\u304c\u5c11\u306a\u3044\u305f\u3081\u30b5\u30f3\u30d7\u30eb\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u884c\u308f\u308c\u306a\u304f\u306a\u308a\u307e\u3059\u3002 \u3053\u306e\u5834\u5408\u306f\u30015X Buffer\u3092\u7528\u3044\u3066\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e020uL\u3067\u53cd\u5fdc\u3059\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3044\u305f\u3057\u307e\u3059\u3002\uff08A\u306e\u6761\u4ef6\uff09 \u53cd\u5fdc\u7cfb\u306e\u5909\u66f4\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u3001\u53cd\u5fdc\u5f8c\u30b5\u30f3\u30d7\u30eb\u306b\u6c3415uL\u3092\u52a0\u3048A\u306e\u6761\u4ef6\u3067\u7cbe\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u30dd\u30a4\u30f3\u30c8\uff1a BigDye Terminator3.1 1\/4\u91cf\u3067\u53cd\u5fdc\u3059\u308b\u5834\u5408\u306f\u3001\u652a\u62cc\u6642\u9593\u3092\u77ed\u304f\u3057\u305f\u307b\u3046\u304c\u826f\u3044\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002 10-20\u5206\u7a0b\u5ea6\u306e\u77ed\u3044\u652a\u62cc\u6642\u9593\u3067\u3082\u30c6\u30b9\u30c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002 \u91cd\u8981\uff1a \u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u30df\u30ad\u30b5\u30fc\u3067\u652a\u62cc\u3059\u308b\u6642\u306f\u300196well Plate\u3082\u3057\u304f\u306f0.2mL\u30c1\u30e5\u30fc\u30d6\u3092\u4f7f\u3044\u307e\u3059\u3002 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