{"id":2204,"date":"2011-10-31T15:37:34","date_gmt":"2011-10-31T06:37:34","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=2204"},"modified":"2011-10-31T15:37:34","modified_gmt":"2011-10-31T06:37:34","slug":"%e6%8a%80%e8%a1%93%e8%ac%9b%e7%bf%92%e4%bc%9amx3000p-qpcr%e3%82%b7%e3%82%b9%e3%83%86%e3%83%a0","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=2204","title":{"rendered":"\u6280\u8853\u8b1b\u7fd2\u4f1a(Mx3000P QPCR\u30b7\u30b9\u30c6\u30e0)"},"content":{"rendered":"<p>\u30a2\u30b8\u30ec\u30f3\u30c8\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u793eMx3000P QPCR\u30b7\u30b9\u30c6\u30e0(Mx3000P\/Mx3005P)\u306b\u95a2\u3059\u308b\u30bb\u30df\u30ca\u30fc\u304a\u3088\u3073\u30c7\u30e2\u30f3\u30b9\u30c8\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u65e5\u6642\uff1a2011\u5e7411\u670807\u65e5(\u6708\u66dc) 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\/>\n\u5b9a\u91cfPCR\u6cd5\u306e\u57fa\u672c\u539f\u7406\u3001\u5b9f\u9a13\u7cfb\u69cb\u7bc9\uff08SYBR or TaqMan prove\u3001\u7d76\u5bfe\u5b9a\u91cf\u6cd5\u3068\u76f8\u5bfe \u5b9a\u91cf\u6cd5\u3001\u76f8\u5bfe\u5b9a\u91cf\uff08\u22bf\u22bfCt\uff09\u6cd5\u306e\u57fa\u672c\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3001cDNA\u5408\u6210\u3001\u30d0\u30a4\u30aa\u30a2\u30ca\u30e9\u30a4\u30b6\u30fc \u306b\u3088\u308bRNA\u306e\u54c1\u8cea\u7ba1\u7406\u3001\u30cf\u30a6\u30b9\u30ad\u30fc\u30d4\u30f3\u30b0\u907a\u4f1d\u5b50\u306e\u9078\u629e\u306a\u3069<\/p>\n<p>\uff3b\u5b9a\u91cfPCR\u6cd5\u306e\u5b9f\u9a13\u7cfb\u69cb\u7bc9\u3000\uff5e\u305d\u306e\u30c7\u30fc\u30bf\u306e\u8ad6\u6587\u63b2\u8f09\u307e\u3067 (MIQE\u30ac\u30a4\u30c9\u30e9\u30a4\u30f3\u306e\u5229\u7528\uff09\uff3d<br \/>\n\u5b9a\u91cfPCR\u6cd5\u306e\u30c7\u30fc\u30bf\u306e\u89e3\u6790\u30fb\u89e3\u91c8\u3001Standard 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