{"id":13617,"date":"1978-11-09T14:02:57","date_gmt":"1978-11-09T05:02:57","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=13617"},"modified":"2020-12-14T16:34:19","modified_gmt":"2020-12-14T07:34:19","slug":"dna%e3%82%b7%e3%83%bc%e3%82%af%e3%82%a8%e3%83%b3%e3%82%b9%e5%8f%97%e8%a8%97%e8%a7%a3%e6%9e%90%e3%82%b5%e3%83%bc%e3%83%93%e3%82%b9%e3%81%ab%e3%81%a4%e3%81%84%e3%81%a6","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=13617","title":{"rendered":"DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u306b\u3064\u3044\u3066"},"content":{"rendered":"

\u5229\u7528\u306b\u3064\u3044\u3066\u306f\u6307\u5c0e\u6559\u54e1\u306e\u627f\u8afe\u3092\u53d7\u3051\u3001Webform(e-mail)\u3067\u7533\u3057\u8fbc\u307f\u3092\u884c\u3044\u307e\u3059\u3002\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u4e0b\u3055\u3044\u3002
\n\u3000
\n\u3000
\n\u25cf \u30b5\u30fc\u30d3\u30b9\u6982\u8981<\/strong><\/p>\n

\u30fb\u53d7\u8a17\u30b5\u30f3\u30d7\u30eb\u6570\u306f8\u30b5\u30f3\u30d7\u30eb\u5358\u4f4d<\/strong><\/span>\u3067\u3059\u3002\u7aef\u6570\u306f\u6b21\u306e\u5358\u4f4d\u6570\u306b\u5408\u308f\u305b\u3066\u8acb\u6c42\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n

\u30fbDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306f\u6708\u30fb\u6c34\u30fb\u6728<\/strong><\/span>\u306e\u90313\u56de\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002\u30c7\u30fc\u30bf\u306e\u9001\u4fe1\u306f\u901a\u5e38\u89e3\u6790\u65e5\u306e\u7fcc\u65e5<\/u>(\u706b\u30fb\u6728\u30fb\u91d1<\/strong><\/span>)\u3067\u3059\u3002(\u4f46\u3057\u3001\u696d\u52d9\u306e\u90fd\u5408\u306b\u3088\u308a\u7d50\u679c\u9001\u4fe1\u304c\u9045\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059)<\/del>\u3000\u4fdd\u7559<\/p>\n

\u30fb<\/strong>ABI3500xl Genetic Analyzer<\/strong><\/a>(\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc24\u672c\u30bf\u30a4\u30d7)\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002
\n\u30fb<\/strong>\u7d042.3\u6642\u9593\u306e\u6cf3\u52d5\u3067\u7d04800\u5869\u57fa(\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u304c\u826f\u597d\u306e\u5834\u5408)\u8aad\u3080\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u30fb<\/strong>\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u53ca\u3073\u6cf3\u52d5\u64cd\u4f5c\u306f\u3059\u3079\u3066\u65bd\u8a2d\u30b9\u30bf\u30c3\u30d5\u304c\u884c\u3044\u307e\u3059\u3002<\/p>\n

\u30fb<\/strong>\u7533\u8acb\u306f”Webform”\u3092\u3054\u5229\u7528\u4e0b\u3055\u3044(\u5b66\u5185\u306e\u307f\u30a2\u30af\u30bb\u30b9\u53ef\u80fd)\u3002
\n\u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_16sample\u4ee5\u4e0b<\/a>
\n\u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_17samples\u4ee5\u4e0a<\/a>
\n\u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_17samples\u4ee5\u4e0a_Excel\u7528<\/a><\/p>\n

\u30fb<\/strong>\u8981\u9818\u306b\u5f93\u3044\u5fc5\u8981\u4e8b\u9805\u3092\u8a18\u5165\u3057\u3001\u78ba\u8a8d\u30dc\u30bf\u30f3\u3092\u62bc\u3059\u3068\u78ba\u8a8d\u753b\u9762\u306b\u306a\u308a\u307e\u3059\u3002\u78ba\u8a8d\u5f8c\u3001\u9001\u4fe1\u3092\u62bc\u3059\u3068\u7ba1\u7406\u5ba4\u5b9b\u306be-mail\u304c\u9001\u4fe1\u3055\u308c\u307e\u3059\u3002
\n\u30fb<\/strong>\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u89e3\u6790\u3092\u958b\u59cb\u3059\u308b\u4e88\u5b9a\u65e5\u3092\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002\u89e3\u6790\u65e5\u306b\u5408\u308f\u305b\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u4e0b\u3055\u3044\u3002\u89e3\u6790\u65e5\u306e\u6307\u5b9a\u306f\u3067\u304d\u307e\u305b\u3093<\/u>\u3002
\n\u30fb<\/strong>\u89e3\u6790\u306e\u9806\u756a\u306f\u5148\u7740\u9806\u3068\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u3000\u3000\u7533\u8acb\u2192\u89e3\u6790\u65e5\u78ba\u8a8d\u2192\u63d0\u51fa<\/span><\/strong> \u306e\u6d41\u308c\u3092\u5b88\u3063\u3066\u4e0b\u3055\u3044\u3002<\/p>\n

\u30fb<\/strong>\u89e3\u6790\u7d42\u4e86\u5f8c\u3001e-mail\u3067\u30c7\u30fc\u30bf\u3092\u5727\u7e2e\u5f8c\u6dfb\u4ed8\u3057\u3066\u9001\u4fe1\u3057\u307e\u3059\u3002<\/p>\n

\u25cf \u63d0\u51fa\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u30fb0.2\u00b5l<\/u><\/strong><\/span>\u306e8\u9023\u30c1\u30e5\u30fc\u30d6\u3067\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002<\/p>\n

\u30fb<\/strong>\u753b\u50cf\u306e\u3088\u3046\u306b\u300c\u7533\u8acb\u8005\u306e\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c83\u6587\u5b57\u300d<\/span><\/strong>\u3068\u300c\u901a\u3057\u756a\u53f7\u300d<\/span><\/strong>\u3092\u8a18\u5165\u3057\u307e\u3059\u3002\u3000\u3000[ ex. \u7530\u6d66 \uff1e TAU ]\n\u30fb<\/strong>\u7aef\u6570\u304c\u3042\u308b\u5834\u5408\u3067\u30828\u9023\u30c1\u30e5\u30fc\u30d6\u306f\u5207\u3089\u305a\u306b\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044<\/strong><\/span>\u3002
\n\u3000
\n\u3000
\n\"\"<\/a>
\n\u3000
\n\u25cf \u8abf\u88fd\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u30fb\u5404\u81ea\u306e\u7814\u7a76\u5ba4\u3067\u306e\u6e96\u5099<\/strong><\/p>\n

\u30fb\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u4f7f\u7528\u3059\u308bTemplate\u306f\u7cbe\u88fd\u6e08\u307f\u306e\u3082\u306e<\/strong><\/span>\u3092\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002<\/p>\n

\u30fbTemplate + Primer + H2O =<\/span> 10\u00b5l<\/strong><\/span><\/u> \u3068\u306a\u308b\u3088\u3046\u30018\u9023\u30c1\u30e5\u30fc\u30d6\u306b\u5206\u6ce8\u3057\u3066\u4e0b\u3055\u3044\u3002
\n(10\u00b5l\u306e\u53cd\u5fdc\u7cfb\u3067\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3044\u307e\u3059\u304c\u300110\u00b5l\u306e\u63d0\u51fa\u5206\u306e\u3046\u30615\u00b5l\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u81ea\u52d5\u5206\u6ce8\u88c5\u7f6e\u3067\u884c\u3063\u3066\u304a\u308a\u3001\u30c1\u30e5\u30fc\u30d6\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u306f\u5b8c\u5168\u306b\u5438\u3048\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306f5\u00b5lx2\u56de\u5206\u306e\u3088\u3046\u306a\u30a4\u30e1\u30fc\u30b8\u3067\u3059\u3002)<\/p>\n

\u3000ex. pGEM32f(+)\u3001Primer M13\u306e\u5834\u5408<\/p>\n

\u3000\u3000\u3000\u3000pGEM32f(+)\u3000200ng\/\u00b5l, Double-stranded DNA(75ng-150ng\u76ee\u5b89)
\n\u3000\u3000 \u3000\u3000\u3000\/\u3000Primer M13\u30000.8pmol\/\u00b5l, Primer(1.6pmol\u76ee\u5b89)<\/p>\n

\u3000\u25b6\u3000( pGEM32f(+) 1.0\u00b5l + Primer M13 2.0\u00b5l + H2O 2.0\u00b5l ) x 2 = 10\u00b5l<\/p>\n

\u30fb\u65bd\u8a2d\u3067\u306f\u3001\u63d0\u51fa\u9802\u3044\u305f8\u9023\u30c1\u30e5\u30fc\u30d6\u306bBigDye Terminator Reaction kit 0.5\u00b5l\u3068x5 Cycle Sequence Buffer 1.5\u00b5l<\/strong><\/span><\/del>(\u4fdd\u7559) \u3092\u52a0\u3048\u3001Final volume 10\u00b5l\u3068\u3057\u3066\u3001\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002
\n\u30fb\u30b5\u30f3\u30d7\u30eb\u306e\u5165\u3063\u3066\u3044\u308b\u30c1\u30e5\u30fc\u30d6\u306b\u3001\u524d\u8ff0\u306e\u4f8b\u306b\u3042\u308b\u3088\u3046\u306b\u3001\u7533\u8acb\u8005\u306e\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c83\u6587\u5b57\u3068\u901a\u3057\u756a\u53f7<\/u>\u3092\u4ed8\u3051\u3066\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002<\/p>\n

\u30fbTemplate\u3068Primer\u306e\u76ee\u5b89\u306f\u4ee5\u4e0b\u306e\u901a\u308a\u3067\u3059(lifetechnologies\u30d7\u30ed\u30c8\u30b3\u30eb\u3088\u308a\u8ee2\u8f09)\u3002
\n\u30fb\u65bd\u8a2d\u3067\u6fc3\u5ea6\u8abf\u6574\u7b49\u306f\u884c\u3044\u307e\u305b\u3093\u3002<\/p>\n

\"\"<\/a>
\n\u3000
\n\u30fb\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u3078\u6301\u53c2\u3059\u308b<\/strong><\/p>\n

1. \u30b5\u30f3\u30d7\u30eb\u30c1\u30e5\u30fc\u30d6\u3092\u65bd\u8a2d\u3067\u7528\u610f\u3057\u3066\u3044\u308b\u5c02\u7528\u30b1\u30fc\u30b9\u306b\u79fb\u3057\u3001\u30ab\u30d0\u30fc\u3092\u3059\u308b\u3002<\/p>\n

2. \u5c02\u7528\u30b1\u30fc\u30b9\u306e\u30ab\u30d0\u30fc\u306b\u65e5\u4ed8\u3001\u6c0f\u540d\u3001\u30b5\u30f3\u30d7\u30eb\u6570\u3092\u66f8\u3044\u305f\u4ed8\u7b8b\u3092\u8cbc\u308a\u3001\u5171\u540c\u5229\u7528\u5b9f\u9a13\u5ba4(301\u53f7\u5ba4)\u306b\u3042\u308b\u51b7\u8535\u5eab\u306e\u6240\u5b9a\u306e\u5834\u6240\u306b\u5165\u308c\u308b\u3002<\/p>\n

\"\"<\/a>
\n\u3000
\n\u3000
\n\u25cf \u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u53ca\u3073\u7cbe\u88fd\u306b\u3064\u3044\u3066(\u65bd\u8a2d\u304c\u5b9f\u65bd)<\/strong><\/p>\n

\u30fb\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u306fFast<\/strong><\/span>\u3067\u884c\u3044\u307e\u3059(\u7d041\u6642\u9593)\u3002
\n\u30fb\u8a66\u85ac\u53ca\u3073\u624b\u6280\u306b\u554f\u984c\u304c\u306a\u3044\u304b\u78ba\u8a8d\u3059\u308b\u305f\u3081\u306b\u3001\u6bce\u56depGEM32f(+)(Primer M13)\u3092Control Sample\u3068\u3057\u3066\u7528\u3044\u3066\u3044\u307e\u3059\u3002<\/p>\n

\"\"<\/a><\/p>\n

\u30fb\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u5f8c\u306e\u7cbe\u88fd\u306f\u4f4e\u6599\u91d1\u30b5\u30fc\u30d3\u30b9\u306e\u5b9f\u73fe\u306e\u305f\u3081<\/u>\u300c\u30a8\u30bf\u30ce\u30fc\u30eb\/EDTA\/\u9162\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\u7cbe\u88fd\u300d<\/strong><\/span>\u3067\u884c\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u300180bp\u4ed8\u8fd1\u306bDyeBlob\u3068\u547c\u3070\u308c\u308b\u86cd\u5149\u8272\u7d20\u306e\u6b8b\u6e23\u304c\u51fa\u307e\u3059\u304c\u3001\u305d\u306e\u5834\u5408\u306f\u4ee5\u4e0b\u306e\u3088\u3046\u306b\u5bfe\u51e6\u3059\u308c\u3070\u554f\u984c\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n

\u2460 Sequence Scanner Software\u306e\u300cAnalyzed\u300d\u30bf\u30d6\u306780bp\u4ed8\u8fd1\u306e\u6ce2\u5f62\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002
\n\u2461 DyeBlob\u306b\u96a0\u308c\u3066\u3044\u308b\u5869\u57fa\u306e\u8272\u3092\u78ba\u8a8d\u3057\u3066\u3001\u8a18\u9332\u3057\u307e\u3059\u3002
\n\u30fb\u96a0\u308c\u3066\u3044\u308bDyeBlob\u3068\u5869\u57fa\u304c\u540c\u8272\u306e\u5834\u5408\u306f\u6ce2\u5f62\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002
\n\u30fb\u56f3\u306fpGEM\u306e\u3082\u306e\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u3053\u306e\u5834\u5408\u306f\u300cGAGTATTC\u300d(74-81bp)\u3068\u5224\u308a\u307e\u3059\u3002
\n\u2462 seq file\u3092\u30c6\u30ad\u30b9\u30c8\u30a8\u30c7\u30a3\u30bf(Notepad\u7b49)\u3067\u958b\u304d\u3001\u8a72\u5f53\u90e8\u5206\u3092\u4fee\u6b63\u3057\u3066\u4fdd\u5b58\u3057\u307e\u3059\u3002
\n\u30fb1\u5217\u304c80\u6587\u5b57\u306a\u306e\u30671\u5217\u76ee\u306e\u53f3\u8fba\u308a\u306b\u306a\u308a\u307e\u3059\u3002
\n\u30fb\u4eca\u56de\u306e\u30b1\u30fc\u30b9\u3067\u306f\u4fee\u6b63\u306e\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\n
\n<\/a>\n<\/div>\n
\n<\/a>\n<\/div>\n<\/div>\n
\n
\n<\/a>\n<\/div>\n
\n<\/a>\n<\/div>\n<\/div>\n

\u3000
\n\u25cf \u30c7\u30fc\u30bf\u306b\u3064\u3044\u3066<\/strong><\/p>\n

\u30fb\u3054\u304f\u7a00\u306b\u3067\u3059\u304c\u3001\u5869\u57fa\u914d\u5217\u89e3\u6790\u7d50\u679c\u3092\u9001\u4ed8\u3057\u305f\u5f8c\u306b\u3001\u5229\u7528\u8005\u304b\u3089\u300cseq\u30d5\u30a1\u30a4\u30eb\u304c\u898b\u5f53\u305f\u3089\u306a\u3044\u306e\u3067\u518d\u5ea6\u9001\u4ed8\u3057\u3066\u4e0b\u3055\u3044\u300d\u3068\u306e\u554f\u3044\u5408\u308f\u305b\u30e1\u30fc\u30eb\u3092\u9802\u304d\u307e\u3059\u3002
\n\u30fb\u901a\u5e38\u3001\u9001\u3089\u308c\u305f\u7d50\u679c\u306e\u4e2d\u306bseq\u30d5\u30a1\u30a4\u30eb\u304c\u7121\u3044\u5834\u5408\u306f\u3001\u89e3\u6790\u7d50\u679c\u304c\u601d\u308f\u3057\u304f\u306a\u304f\u3001\u914d\u5217\u3068\u3057\u3066\u6271\u3046\u3053\u3068\u304c\u3067\u304d\u306a\u304b\u3063\u305f\u5834\u5408\u304c\u307b\u3068\u3093\u3069\u3067\u3059\u3002\u305d\u306e\u5834\u5408\u306f\u3001\u9069\u5207\u306a\u89e3\u6790\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3057\u3066\u30af\u30ed\u30de\u30c8(\u6ce2\u5f62)\u306e\u30d5\u30a1\u30a4\u30eb\u3092\u8868\u793a\u3059\u308b\u3068\u3001\u539f\u56e0\u3092\u660e\u3089\u304b\u306b\u3059\u308b\u4e00\u52a9\u3068\u306a\u308b\u304b\u3068\u601d\u308f\u308c\u307e\u3059\u3002<\/p>\n

\u30fb\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u306eHP\u306eContents\u5185\u306b\u3001\u300c13 \u5b9f\u9a13\u30fb\u5229\u7528\u6cd5\u300d\u3068\u3044\u3046\u30da\u30fc\u30b8\u304c\u3042\u308a\u3001\u305d\u306e\u4e2d\u306b\u300cDNA\u30b7\u30fc\u30b1\u30f3\u30b9\u6ce2\u5f62\u30c7\u30fc\u30bf\u7de8\u96c6\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3064\u3044\u3066<\/strong><\/a>\u300d\u3068\u3044\u3046\u8a18\u4e8b\u304c\u3042\u308a\u307e\u3059\u3002
\n\u30fbMAC OS\u3001WINDOWS\u306b\u5bfe\u5fdc\u3057\u305f\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306e\u60c5\u5831\u3092\u8f09\u305b\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u74b0\u5883\u306b\u3042\u3063\u305f\u3082\u306e\u3092\u3054\u4f7f\u7528\u4e0b\u3055\u3044\u3002
\n\u30fbWINDOWS\u3067\u3042\u308c\u3070Applied Biosystems\u7d14\u6b63\u306eSequence Scanner Software\u304c\u4f7f\u3044\u3084\u3059\u3044\u3068\u601d\u308f\u308c\u307e\u3059\u3002\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u4f7f\u7528\u3059\u308c\u3070ab1\u30d5\u30a1\u30a4\u30eb\u3088\u308a\u6ce2\u5f62\u30c7\u30fc\u30bf\u3092\u53c2\u7167\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059(Windows XP\u4ee5\u5916\u306b\u3082Windows7\u3068Windows10\u3067\u52d5\u4f5c\u3092\u78ba\u8a8d\u3057\u3066\u3044\u307e\u3059)\u3002
\n\u30fbMAC\u3067\u3082Sequence Scanner Software\u3092\u30a4\u30f3\u30b9\u30c8\u30fc\u30eb\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u8a73\u7d30\u306f\u300cMac\u5411\u3051Sequence Scanner\/Peak Scanner \u5c0e\u5165\u30de\u30cb\u30e5\u30a2\u30eb2_PlayOnMac_kagoshima-u ver.<\/strong><\/a>\u300d\u3092\u53c2\u7167\u3057\u3066\u4e0b\u3055\u3044\u3002
\n\u30fb\u30af\u30ed\u30de\u30c8\u30c7\u30fc\u30bf\u306b\u3064\u3044\u3066\u3054\u4e0d\u660e\u306a\u70b9\u304c\u3042\u308a\u307e\u3057\u305f\u3089\u3001\u65bd\u8a2d\u307e\u3067\u304a\u6c17\u8efd\u306b\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002
\n\u3000
\n\u3000
\n\u25cf \u6599\u91d1\u7b49\u306b\u3064\u3044\u3066<\/strong><\/p>\n

DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790\u6599\u91d1
\n(\u5b66\u5185\u5229\u7528\u8005)\u30001\u30b5\u30f3\u30d7\u30eb\u3000XXX\u5186<\/del><\/strong><\/span>\u3000\uff0a2021\u5e744\u6708\u6599\u91d1\u8a2d\u5b9a\u4e88\u5b9a<\/p>\n

\u3000
\n\u25cf \u95a2\u9023\u60c5\u5831<\/strong><\/p>\n

\u3000DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc ABI PRISM 3500xl Genetic Analyzer<\/a>
\n\u3000DNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b5\u30fc ABI PRISM 3130xl Genetic Analyzer<\/a>
\n\u3000\u9e7f\u5150\u5cf6\u5927\u5b66\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2dDNA\u89e3\u6790\u521d\u5fc3\u8005\u5411\u3051\u6280\u8853\u8b1b\u7fd2\u4f1a\u7528\u30c6\u30ad\u30b9\u30c8<\/a>
\n\u3000BigDye XTerminator \u7cbe\u88fd\u30ad\u30c3\u30c8\u306b\u3064\u3044\u3066<\/a>
\n\u3000BigDye XTerminator \u7cbe\u88fd\u30ad\u30c3\u30c8\u306b\u3064\u3044\u3066 2<\/a>
\n\u3000DNA\u30b7\u30fc\u30b1\u30f3\u30b9 \u30d7\u30ed\u30c8\u30b3\u30fc\u30eb<\/a>
\n\u3000DNA\u30b7\u30fc\u30b1\u30f3\u30b9\u6ce2\u5f62\u30c7\u30fc\u30bf\u7de8\u96c6\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u306b\u3064\u3044\u3066<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"

\u5229\u7528\u306b\u3064\u3044\u3066\u306f\u6307\u5c0e\u6559\u54e1\u306e\u627f\u8afe\u3092\u53d7\u3051\u3001Webform(e-mail)\u3067\u7533\u3057\u8fbc\u307f\u3092\u884c\u3044\u307e\u3059\u3002\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u4e0b\u3055\u3044\u3002 \u3000 \u3000 \u25cf \u30b5\u30fc\u30d3\u30b9\u6982\u8981 \u30fb\u53d7\u8a17\u30b5\u30f3\u30d7\u30eb\u6570\u306f8\u30b5\u30f3\u30d7\u30eb\u5358\u4f4d\u3067\u3059\u3002\u7aef\u6570\u306f\u6b21\u306e\u5358\u4f4d\u6570\u306b\u5408\u308f\u305b\u3066\u8acb\u6c42\u3044\u305f\u3057\u307e\u3059\u3002 \u30fbDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u89e3\u6790\u30b5\u30fc\u30d3\u30b9\u306f\u6708\u30fb\u6c34\u30fb\u6728\u306e\u90313\u56de\u5b9f\u65bd\u3057\u3066\u3044\u307e\u3059\u3002\u30c7\u30fc\u30bf\u306e\u9001\u4fe1\u306f\u901a\u5e38\u89e3\u6790\u65e5\u306e\u7fcc\u65e5(\u706b\u30fb\u6728\u30fb\u91d1)\u3067\u3059\u3002(\u4f46\u3057\u3001\u696d\u52d9\u306e\u90fd\u5408\u306b\u3088\u308a\u7d50\u679c\u9001\u4fe1\u304c\u9045\u308c\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059)\u3000\u4fdd\u7559 \u30fbABI3500xl Genetic Analyzer(\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc24\u672c\u30bf\u30a4\u30d7)\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002 \u30fb\u7d042.3\u6642\u9593\u306e\u6cf3\u52d5\u3067\u7d04800\u5869\u57fa(\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u304c\u826f\u597d\u306e\u5834\u5408)\u8aad\u3080\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002 \u30fb\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53cd\u5fdc\u53ca\u3073\u6cf3\u52d5\u64cd\u4f5c\u306f\u3059\u3079\u3066\u65bd\u8a2d\u30b9\u30bf\u30c3\u30d5\u304c\u884c\u3044\u307e\u3059\u3002 \u30fb\u7533\u8acb\u306f”Webform”\u3092\u3054\u5229\u7528\u4e0b\u3055\u3044(\u5b66\u5185\u306e\u307f\u30a2\u30af\u30bb\u30b9\u53ef\u80fd)\u3002 \u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_16sample\u4ee5\u4e0b \u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_17samples\u4ee5\u4e0a \u3000\u25cfDNA\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u53d7\u8a17\u89e3\u6790 Premix\u30b5\u30fc\u30d3\u30b9\u5229\u7528\u7533\u8acb_17samples\u4ee5\u4e0a_Excel\u7528 \u30fb\u8981\u9818\u306b\u5f93\u3044\u5fc5\u8981\u4e8b\u9805\u3092\u8a18\u5165\u3057\u3001\u78ba\u8a8d\u30dc\u30bf\u30f3\u3092\u62bc\u3059\u3068\u78ba\u8a8d\u753b\u9762\u306b\u306a\u308a\u307e\u3059\u3002\u78ba\u8a8d\u5f8c\u3001\u9001\u4fe1\u3092\u62bc\u3059\u3068\u7ba1\u7406\u5ba4\u5b9b\u306be-mail\u304c\u9001\u4fe1\u3055\u308c\u307e\u3059\u3002 \u30fb\u7533\u8acb\u306e\u53d7\u4ed8\u5f8c\u3001\u89e3\u6790\u3092\u958b\u59cb\u3059\u308b\u4e88\u5b9a\u65e5\u3092\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002\u89e3\u6790\u65e5\u306b\u5408\u308f\u305b\u3066\u30b5\u30f3\u30d7\u30eb\u3092\u304a\u6301\u3061\u4e0b\u3055\u3044\u3002\u89e3\u6790\u65e5\u306e\u6307\u5b9a\u306f\u3067\u304d\u307e\u305b\u3093\u3002 \u30fb\u89e3\u6790\u306e\u9806\u756a\u306f\u5148\u7740\u9806\u3068\u306a\u308a\u307e\u3059\u3002 \u3000\u3000\u7533\u8acb\u2192\u89e3\u6790\u65e5\u78ba\u8a8d\u2192\u63d0\u51fa \u306e\u6d41\u308c\u3092\u5b88\u3063\u3066\u4e0b\u3055\u3044\u3002 \u30fb\u89e3\u6790\u7d42\u4e86\u5f8c\u3001e-mail\u3067\u30c7\u30fc\u30bf\u3092\u5727\u7e2e\u5f8c\u6dfb\u4ed8\u3057\u3066\u9001\u4fe1\u3057\u307e\u3059\u3002 \u25cf \u63d0\u51fa\u30b5\u30f3\u30d7\u30eb\u306b\u3064\u3044\u3066 \u30fb0.2\u00b5l\u306e8\u9023\u30c1\u30e5\u30fc\u30d6\u3067\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002 \u30fb\u753b\u50cf\u306e\u3088\u3046\u306b\u300c\u7533\u8acb\u8005\u306e\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c83\u6587\u5b57\u300d\u3068\u300c\u901a\u3057\u756a\u53f7\u300d\u3092\u8a18\u5165\u3057\u307e\u3059\u3002\u3000\u3000[ ex. \u7530\u6d66 \uff1e TAU ] \u30fb\u7aef\u6570\u304c\u3042\u308b\u5834\u5408\u3067\u30828\u9023\u30c1\u30e5\u30fc\u30d6\u306f\u5207\u3089\u305a\u306b\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002 \u3000 \u3000 \u3000 \u25cf \u8abf\u88fd\u306b\u3064\u3044\u3066 \u30fb\u5404\u81ea\u306e\u7814\u7a76\u5ba4\u3067\u306e\u6e96\u5099 \u30fb\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u306b\u4f7f\u7528\u3059\u308bTemplate\u306f\u7cbe\u88fd\u6e08\u307f\u306e\u3082\u306e\u3092\u63d0\u51fa\u3057\u3066\u4e0b\u3055\u3044\u3002 \u30fbTemplate + Primer + H2O = 10\u00b5l \u3068\u306a\u308b\u3088\u3046\u30018\u9023\u30c1\u30e5\u30fc\u30d6\u306b\u5206\u6ce8\u3057\u3066\u4e0b\u3055\u3044\u3002 (10\u00b5l\u306e\u53cd\u5fdc\u7cfb\u3067\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30af\u30a8\u30f3\u30b9\u3092\u884c\u3044\u307e\u3059\u304c\u300110\u00b5l\u306e\u63d0\u51fa\u5206\u306e\u3046\u30615\u00b5l\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002\u81ea\u52d5\u5206\u6ce8\u88c5\u7f6e\u3067\u884c\u3063\u3066\u304a\u308a\u3001\u30c1\u30e5\u30fc\u30d6\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u306f\u5b8c\u5168\u306b\u5438\u3048\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306f5\u00b5lx2\u56de\u5206\u306e\u3088\u3046\u306a\u30a4\u30e1\u30fc\u30b8\u3067\u3059\u3002) \u3000ex. pGEM32f(+)\u3001Primer M13\u306e\u5834\u5408 \u3000\u3000\u3000\u3000pGEM32f(+)\u3000200ng\/\u00b5l, [Continue]<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[3],"tags":[],"class_list":["post-13617","post","type-post","status-publish","format-standard","hentry","category-dna"],"_links":{"self":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/13617","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=13617"}],"version-history":[{"count":109,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/13617\/revisions"}],"predecessor-version":[{"id":14368,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/13617\/revisions\/14368"}],"wp:attachment":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=13617"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=13617"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=13617"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}