{"id":10988,"date":"2018-06-29T15:21:06","date_gmt":"2018-06-29T06:21:06","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=10988"},"modified":"2018-06-29T16:04:18","modified_gmt":"2018-06-29T07:04:18","slug":"%e6%8a%80%e8%a1%93%e8%ac%9b%e7%bf%92%e4%bc%9a%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr-%e5%9f%ba%e7%a4%8e%e3%82%bb%e3%83%9f%e3%83%8a%e3%83%bc-2","status":"publish","type":"post","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?p=10988","title":{"rendered":"\u6280\u8853\u8b1b\u7fd2\u4f1a(\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR \u57fa\u790e\u30bb\u30df\u30ca\u30fc)"},"content":{"rendered":"<p>\u30e9\u30a4\u30d5\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u30ba\u30b8\u30e3\u30d1\u30f3\u793e\u3000\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u95a2\u3059\u308b\u57fa\u790e\u30bb\u30df\u30ca\u30fc\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u65e5\u6642\uff1a2018\u5e747\u670820\u65e5(\u91d1)\u300013:00\uff5e16:00<\/li>\n<li>\u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3000\u30bb\u30df\u30ca\u30fc\u5ba4(1F)<\/li>\n<li>\u62c5\u5f53\uff1a\u30e9\u30a4\u30d5\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u30ba\u30b8\u30e3\u30d1\u30f3\u682a\u5f0f\u4f1a\u793e\u3000\u767d\u795e \u535a<\/li>\n<li>\u6f14\u984c1\uff1a\u826f\u597d\u306a\u30c7\u30fc\u30bf\u3092\u51fa\u3059\u305f\u3081\u306e\u30dd\u30a4\u30f3\u30c8 &#038; miRNA\u30fb\u5909\u7570\u89e3\u6790<\/li>\n<li>\u6f14\u984c2\uff1aStepOnePlus \u5b9f\u6a5f\u53d6\u6271\u8aac\u660e\u4f1a<\/li>\n<p>\u3000<\/p>\n<li>\u6982\u8981<\/li>\n<p>\u25cf \u7b2c1\u90e8\uff1a\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u7528\u3044\u305fStepOnePlus\u306e\u4f7f\u7528\u8aac\u660e\u4f1a<\/p>\n<p>\u30c1\u30e5\u30fc\u30c8\u30ea\u30a2\u30eb\u8cc7\u6599\u3092\u7528\u3044\u305f\u4f7f\u7528\u8aac\u660e\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002\u7b2c2\u90e8\u3068\u5408\u308f\u305b\u3066\u8074\u8b1b\u9802\u304f\u3053\u3068\u3067\u826f\u597d\u306a\u7d50\u679c\u3092\u5f97\u308b\u65b9\u6cd5\u3092\u7406\u89e3\u3057\u305f\u308a\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u5b9f\u9a13\u64cd\u4f5c\u3092\u59cb\u3081\u308b\u65b9\u306b\u6709\u7528\u306a\u8aac\u660e\u4f1a\u3067\u3059\u3002<\/p>\n<p>\u25cf \u7b2c2\u90e8\uff1a\u57fa\u790e\u539f\u7406\u3068\u6d3b\u7528\u6cd5\u7d39\u4ecb<\/p>\n<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306fmRNA\u767a\u73fe\u89e3\u6790\u3067\u591a\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u6bb5\u968e\u304b\u3089\u6ce8\u610f\u3059\u308b\u3079\u304d\u70b9\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u9078\u629e\u3084\u0394\u0394Ct\u6cd5\u306a\u3069\u306e\u89e3\u6790\u65b9\u6cd5\u3001\u8a66\u85ac\u6df7\u5408\u306a\u3069\u591a\u6570\u306e\u30b3\u30c4\u3084\u898b\u843d\u3068\u3057\u304c\u3061\u306a\u70b9\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306e\u30bb\u30df\u30ca\u30fc\u3067\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u304b\u3089\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8\u307e\u3067\u5177\u4f53\u7684\u306a\u4e8b\u4f8b\u3092\u542b\u3081\u3066\u7d39\u4ecb\u3057\u3001\u826f\u597d\u306a\u7d50\u679c\u3092\u51fa\u3059\u305f\u3081\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n<p>\u307e\u305f\u3001\u6d3b\u7528\u7de8\u3067\u306fmicroRNA\u306e\u767a\u73fe\u89e3\u6790\u3084SNP\u306a\u3069\u306e\u30b2\u30ce\u30e0\u5909\u7570\u89e3\u6790\u306e\u30dd\u30a4\u30f3\u30c8\u3082\u63d0\u4f9b\u3057\u307e\u3059\u3002\u73fe\u5728\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u304a\u4f7f\u3044\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u59cb\u3081\u305f\u3044\u65b9\u306a\u3069\u306b\u6700\u9069\u306a\u5185\u5bb9\u3092\u542b\u307f\u307e\u3059\u306e\u3067\u3001\u3053\u306e\u6a5f\u4f1a\u306b\u662f\u975e\u3054\u53c2\u52a0\u4e0b\u3055\u3044\u3002<\/p>\n<\/li>\n<li>\u5185\u5bb9<\/li>\n<p>\u30fb13:00\uff5e14:20\u3000\u7b2c1\u90e8\u3000StepOnePlus\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u4f7f\u7528\u8aac\u660e\u4f1a<br \/>\n\u30fb14:30\uff5e16:00\u3000\u7b2c2\u90e8\u3000\u57fa\u790e\u539f\u7406\u3068\u6d3b\u7528\u6cd5\u7d39\u4ecb<\/p>\n<li>\u7533\u3057\u8fbc\u307f<\/li>\n<p>\u7533\u3057\u8fbc\u307f\u306f\u7533\u8fbc\u30d5\u30a9\u30fc\u30e0\u304b\u3089\u884c\u3044\u307e\u3059\u3002\u3000<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=4195\" target=\"_blank\"> <strong><span style=\"color: #006400;\">\u25b6\u3000&#8221;\u30bb\u30df\u30ca\u30fc\u7533\u8fbc&#8221;<\/span><\/strong> <\/a><br \/>\n\u30bb\u30df\u30ca\u30fc\u306e\u7533\u3057\u8fbc\u307f\u306f\u5fc5\u9808\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u7533\u3057\u8fbc\u307f\u3092\u3057\u306a\u304b\u3063\u305f\u5834\u5408\u3001\u60f3\u5b9a\u4ee5\u4e0a\u306e\u53c2\u52a0\u304c\u3042\u3063\u305f\u3068\u304d\u306b\u8cc7\u6599\u3092\u6e96\u5099\u3067\u304d\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059(\u8cc7\u6599\u306e\u30b3\u30d4\u30fc\u7b49\u306f\u884c\u3044\u307e\u305b\u3093)\u3002\n<\/ul>\n<ul>\n<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2018\/06\/20180720_realtimepcr-seminar.pdf\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2018\/06\/20180720_realtimepcr-seminar1.png\" alt=\"20180720_realtime-seminar\" width=\"207\" height=\"288\" class=\"aligncenter size-full wp-image-10992\" \/><\/a>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u30e9\u30a4\u30d5\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u30ba\u30b8\u30e3\u30d1\u30f3\u793e\u3000\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306b\u95a2\u3059\u308b\u57fa\u790e\u30bb\u30df\u30ca\u30fc\u306e\u958b\u50ac\u306b\u3064\u3044\u3066\u304a\u77e5\u3089\u305b\u3057\u307e\u3059\u3002 \u65e5\u6642\uff1a2018\u5e747\u670820\u65e5(\u91d1)\u300013:00\uff5e16:00 \u5834\u6240\uff1a\u907a\u4f1d\u5b50\u5b9f\u9a13\u65bd\u8a2d\u3000\u30bb\u30df\u30ca\u30fc\u5ba4(1F) \u62c5\u5f53\uff1a\u30e9\u30a4\u30d5\u30c6\u30af\u30ce\u30ed\u30b8\u30fc\u30ba\u30b8\u30e3\u30d1\u30f3\u682a\u5f0f\u4f1a\u793e\u3000\u767d\u795e \u535a \u6f14\u984c1\uff1a\u826f\u597d\u306a\u30c7\u30fc\u30bf\u3092\u51fa\u3059\u305f\u3081\u306e\u30dd\u30a4\u30f3\u30c8 &#038; miRNA\u30fb\u5909\u7570\u89e3\u6790 \u6f14\u984c2\uff1aStepOnePlus \u5b9f\u6a5f\u53d6\u6271\u8aac\u660e\u4f1a \u3000 \u6982\u8981 \u25cf \u7b2c1\u90e8\uff1a\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u3092\u7528\u3044\u305fStepOnePlus\u306e\u4f7f\u7528\u8aac\u660e\u4f1a \u30c1\u30e5\u30fc\u30c8\u30ea\u30a2\u30eb\u8cc7\u6599\u3092\u7528\u3044\u305f\u4f7f\u7528\u8aac\u660e\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002\u7b2c2\u90e8\u3068\u5408\u308f\u305b\u3066\u8074\u8b1b\u9802\u304f\u3053\u3068\u3067\u826f\u597d\u306a\u7d50\u679c\u3092\u5f97\u308b\u65b9\u6cd5\u3092\u7406\u89e3\u3057\u305f\u308a\u3001\u30c7\u30fc\u30bf\u89e3\u6790\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u5b9f\u9a13\u64cd\u4f5c\u3092\u59cb\u3081\u308b\u65b9\u306b\u6709\u7528\u306a\u8aac\u660e\u4f1a\u3067\u3059\u3002 \u25cf \u7b2c2\u90e8\uff1a\u57fa\u790e\u539f\u7406\u3068\u6d3b\u7528\u6cd5\u7d39\u4ecb \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306fmRNA\u767a\u73fe\u89e3\u6790\u3067\u591a\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306e\u6bb5\u968e\u304b\u3089\u6ce8\u610f\u3059\u308b\u3079\u304d\u70b9\u304c\u591a\u6570\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u9078\u629e\u3084\u0394\u0394Ct\u6cd5\u306a\u3069\u306e\u89e3\u6790\u65b9\u6cd5\u3001\u8a66\u85ac\u6df7\u5408\u306a\u3069\u591a\u6570\u306e\u30b3\u30c4\u3084\u898b\u843d\u3068\u3057\u304c\u3061\u306a\u70b9\u3082\u3042\u308a\u307e\u3059\u3002 \u4eca\u56de\u306e\u30bb\u30df\u30ca\u30fc\u3067\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u304b\u3089\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8\u307e\u3067\u5177\u4f53\u7684\u306a\u4e8b\u4f8b\u3092\u542b\u3081\u3066\u7d39\u4ecb\u3057\u3001\u826f\u597d\u306a\u7d50\u679c\u3092\u51fa\u3059\u305f\u3081\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u8aac\u660e\u3057\u307e\u3059\u3002 \u307e\u305f\u3001\u6d3b\u7528\u7de8\u3067\u306fmicroRNA\u306e\u767a\u73fe\u89e3\u6790\u3084SNP\u306a\u3069\u306e\u30b2\u30ce\u30e0\u5909\u7570\u89e3\u6790\u306e\u30dd\u30a4\u30f3\u30c8\u3082\u63d0\u4f9b\u3057\u307e\u3059\u3002\u73fe\u5728\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u304a\u4f7f\u3044\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u59cb\u3081\u305f\u3044\u65b9\u306a\u3069\u306b\u6700\u9069\u306a\u5185\u5bb9\u3092\u542b\u307f\u307e\u3059\u306e\u3067\u3001\u3053\u306e\u6a5f\u4f1a\u306b\u662f\u975e\u3054\u53c2\u52a0\u4e0b\u3055\u3044\u3002 \u5185\u5bb9 \u30fb13:00\uff5e14:20\u3000\u7b2c1\u90e8\u3000StepOnePlus\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u4f7f\u7528\u8aac\u660e\u4f1a \u30fb14:30\uff5e16:00\u3000\u7b2c2\u90e8\u3000\u57fa\u790e\u539f\u7406\u3068\u6d3b\u7528\u6cd5\u7d39\u4ecb \u7533\u3057\u8fbc\u307f \u7533\u3057\u8fbc\u307f\u306f\u7533\u8fbc\u30d5\u30a9\u30fc\u30e0\u304b\u3089\u884c\u3044\u307e\u3059\u3002\u3000 \u25b6\u3000&#8221;\u30bb\u30df\u30ca\u30fc\u7533\u8fbc&#8221; \u30bb\u30df\u30ca\u30fc\u306e\u7533\u3057\u8fbc\u307f\u306f\u5fc5\u9808\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u7533\u3057\u8fbc\u307f\u3092\u3057\u306a\u304b\u3063\u305f\u5834\u5408\u3001\u60f3\u5b9a\u4ee5\u4e0a\u306e\u53c2\u52a0\u304c\u3042\u3063\u305f\u3068\u304d\u306b\u8cc7\u6599\u3092\u6e96\u5099\u3067\u304d\u306a\u3044\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059(\u8cc7\u6599\u306e\u30b3\u30d4\u30fc\u7b49\u306f\u884c\u3044\u307e\u305b\u3093)\u3002<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4,5,8],"tags":[],"class_list":["post-10988","post","type-post","status-publish","format-standard","hentry","category-realtime-pcr","category-5","category-8"],"_links":{"self":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/10988","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=10988"}],"version-history":[{"count":7,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/10988\/revisions"}],"predecessor-version":[{"id":11604,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/posts\/10988\/revisions\/11604"}],"wp:attachment":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=10988"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=10988"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=10988"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}