{"id":6655,"date":"2014-06-30T16:25:16","date_gmt":"2014-06-30T07:25:16","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=6655"},"modified":"2025-07-28T16:51:01","modified_gmt":"2025-07-28T07:51:01","slug":"%e5%8c%96%e5%ad%a6%e7%99%ba%e5%85%89%e6%92%ae%e5%bd%b1%e8%a3%85%e7%bd%ae-ez-capture-mg","status":"publish","type":"page","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=6655","title":{"rendered":"\u5316\u5b66\u767a\u5149\u64ae\u5f71\u88c5\u7f6e Ez-Capture MG"},"content":{"rendered":"<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-first \">\n<a href=\".\/wp-content\/uploads\/2014\/06\/ez-capturemg-1.png\" title=\"ez-capturemg-1\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2014\/06\/ez-capturemg-1.png\" title=\"ez-capturemg-1\" width=\"180\" height=\"287\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-7626\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2014\/06\/ez-capturemg-1.png 258w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2014\/06\/ez-capturemg-1-189x300.png 189w\" sizes=\"auto, (max-width: 180px) 100vw, 180px\" \/><\/a>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-last \">\n\u3000<br \/>\n\u3000<br \/>\n\u3000<br \/>\n<a href=\".\/wp-content\/uploads\/2014\/06\/ez-capturemg_fig01-1.png\" title=\"ez-capturemg_fig01-1\" target=\"_blank\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2014\/06\/ez-capturemg_fig01-1.png\" title=\"ez-capturemg_fig01-1\" width=\"217\" height=\"131\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-7627\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2014\/06\/ez-capturemg_fig01-1.png 870w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2014\/06\/ez-capturemg_fig01-1-300x182.png 300w\" sizes=\"auto, (max-width: 217px) 100vw, 217px\" \/><\/a>\n<\/div>\n<\/div>\n<p>\u9ad8\u611f\u5ea6\u306e140\u4e07\u753b\u7d20\u51b7\u5374CCD\u30ab\u30e1\u30e9\u3092\u642d\u8f09\u3057\u305f\u5316\u5b66\u767a\u5149\u64ae\u5f71\u88c5\u7f6e<\/p>\n<p>\u3010\u4ed5\u69d8\u3011<br \/>\n\u6a5f\u7a2e \uff1aEz-Capture MG<br \/>\n\u30e1\u30fc\u30ab\u30fc \uff1a<a href=\"http:\/\/www.atto.co.jp\/\" title=\"ATTO\" target=\"_blank\">ATTO<\/a><br \/>\n\u753b\u7d20\u6570 \uff1a140\u4e07\u753b\u7d20<br \/>\n\u51b7\u5374\u6e29\u5ea6 \uff1a-30\u2103<br \/>\n\u6a5f\u80fd \uff1a\u81ea\u52d5\u9732\u5149\u30fb\u7a4d\u7b97\u9732\u5149\u30fb\u30a4\u30f3\u30bf\u30fc\u30d0\u30eb\u9732\u5149\u6a5f\u80fd\u300116bit TIFF\u4fdd\u5b58\u3001<br \/>\n\u3000\u3000 \uff1a\u81ea\u52d5\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u30fb\u767d\u9ed2\u53cd\u8ee2\u6a5f\u80fd<br \/>\n\u5316\u5b66\u767a\u5149\u64ae\u5f71\uff1a<br \/>\n\u3000\uff3b\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\uff3dHRP\u7528\u767a\u5149\u57fa\u8cea\uff08ECL, EzWestLumi\/plus\u306a\u3069\uff09<br \/>\n\u3000\uff3b\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u3001\u30ce\u30fc\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\uff3dALP\u7528\u767a\u5149\u57fa\u8cea\uff08CSPD, CDP-star\u306a\u3069\uff09<br \/>\n\u86cd\u5149\u64ae\u5f71\uff1a<br \/>\n\u3000\u3000GFP\u7528\u86cd\u5149\u7269\u8cea\uff3bBFP510 filter \/ 1\u6b21\u30d5\u30a3\u30eb\u30bf\u30fc\u30bb\u30c3\u30c8(481nm short pass)\uff3d<br \/>\n\u4ed8\u5c5e\u88c5\u7f6e \uff1aVarirays\uff08\u86cd\u5149\u52b1\u8d77\u7528\u843d\u5c04\u5f0fLED\u5149\u6e90\u30e6\u30cb\u30c3\u30c8\uff09<br \/>\n\u4ed8\u5c5e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2 \uff1aImageSaver6\uff08\u5236\u5fa1\u7528\u30bd\u30d5\u30c8\uff09<\/p>\n<p>\u3010\u8a2d\u7f6e\u5834\u6240\u3011<br \/>\n\u8a66\u6599\u5206\u6790\u5ba4\uff08<a href=\".\/wp-content\/uploads\/2007\/08\/307_20170515.png\" title=\"3\u968e\u3000303\u53f7\u5ba4\" target=\"_blank\">3\u968e\u3000307\u53f7\u5ba4<\/a>\uff09<\/p>\n<p>\u3010\u6982\u8981\u3011<br \/>\n\u30fbCCD\u30ab\u30e1\u30e9\u3092-30\u2103\u51b7\u5374\u3059\u308b\u3053\u3068\u3067\u30ce\u30a4\u30ba\u3092\u4f4e\u6e1b<br \/>\n\u30fb\u64ae\u5f71\u6642\u306b\u30c0\u30fc\u30af\u30a4\u30e1\u30fc\u30b8\u3092\u6e1b\u7b97\u3057\u3001\u66f4\u306b\u30ce\u30a4\u30ba\u4f4e\u6e1b\u51e6\u7406<br \/>\n\u30fb\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u3088\u304f\u5229\u7528\u3055\u308c\u308b\u6709\u8272\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u50cf\u3068\u767a\u5149\u753b\u50cf\u306e\u5408\u6210\u3082\u53ef\u80fd<\/p>\n<p>\u3010\u6a5f\u5668\u5229\u7528\u6599\u91d1\u5bfe\u8c61\u3011<br \/>\n<a title=\"\u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\" href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=22393\" target=\"_blank\" rel=\"noopener noreferrer\">\u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044<\/a><\/p>\n<p>\u3010\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u5bfe\u8c61\u3011<br \/>\n\u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f<span style=\"color: #ff0000;\">\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8<\/span>\u304c\u5fc5\u8981\u3067\u3059\uff08<span style=\"text-decoration: underline;\">\u5404\u7814\u7a76\u5ba41\u3064\u3001\u7814\u7a76\u4ee3\u8868\u8005\u304c\u7533\u8acb<\/span>\uff09\u3002\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u3010\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u5bfe\u8c61\u3011<br \/>\n\u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f<span style=\"color: #0000ff;\">\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0<\/span>\u3078\u306e\u767b\u9332\u304c\u5fc5\u8981\u3067\u3059\uff08<span style=\"text-decoration: underline;\">\u5229\u7528\u8005\u500b\u3005\u304c\u7533\u8acb<\/span>\uff09\u3002\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u306e\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u3010\u5229\u7528\u4f8b\u3011<br \/>\n\u30fb\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u30ce\u30fc\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0(\u73fe\u5728\u306fGFP\u306e\u307f)<\/p>\n<p>\u30fb\u4ee5\u4e0b\u306f\u30aa\u30d7\u30b7\u30e7\u30f3<br \/>\n\u3000\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u86cd\u5149\u67d3\u8272\uff08SYPRO Ruby, Pro-Q Diamond, Oriole\u306a\u3069\uff09\u3001<br \/>\n\u3000\u6838\u9178\u306e\u86cd\u5149\u67d3\u8272\uff08\u30a8\u30c1\u30b8\u30a6\u30e0\u30d6\u30ed\u30de\u30a4\u30c9, SYBR Green\/Safe\u306a\u3069\uff09\u3001<br \/>\n\u3000\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08GFP, RFP\u306a\u3069\uff09\u3001\u8272\u7d20\u67d3\u8272\uff08CBB\u67d3\u8272\u306a\u3069\uff09<br \/>\n\u3000\uff0a\u4e0a\u8a18\u306e\u86cd\u5149\u64ae\u5f71\u3092\u5e0c\u671b\u3055\u308c\u308b\u5834\u5408\u306f\u3001\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000 \u3000 \u3000 \u9ad8\u611f\u5ea6\u306e140\u4e07\u753b\u7d20\u51b7\u5374CCD\u30ab\u30e1\u30e9\u3092\u642d\u8f09\u3057\u305f\u5316\u5b66\u767a\u5149\u64ae\u5f71\u88c5\u7f6e \u3010\u4ed5\u69d8\u3011 \u6a5f\u7a2e \uff1aEz-Capture MG \u30e1\u30fc\u30ab\u30fc \uff1aATTO \u753b\u7d20\u6570 \uff1a140\u4e07\u753b\u7d20 \u51b7\u5374\u6e29\u5ea6 \uff1a-30\u2103 \u6a5f\u80fd \uff1a\u81ea\u52d5\u9732\u5149\u30fb\u7a4d\u7b97\u9732\u5149\u30fb\u30a4\u30f3\u30bf\u30fc\u30d0\u30eb\u9732\u5149\u6a5f\u80fd\u300116bit TIFF\u4fdd\u5b58\u3001 \u3000\u3000 \uff1a\u81ea\u52d5\u30b3\u30f3\u30c8\u30e9\u30b9\u30c8\u30fb\u767d\u9ed2\u53cd\u8ee2\u6a5f\u80fd \u5316\u5b66\u767a\u5149\u64ae\u5f71\uff1a \u3000\uff3b\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\uff3dHRP\u7528\u767a\u5149\u57fa\u8cea\uff08ECL, EzWestLumi\/plus\u306a\u3069\uff09 \u3000\uff3b\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u3001\u30ce\u30fc\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\uff3dALP\u7528\u767a\u5149\u57fa\u8cea\uff08CSPD, CDP-star\u306a\u3069\uff09 \u86cd\u5149\u64ae\u5f71\uff1a \u3000\u3000GFP\u7528\u86cd\u5149\u7269\u8cea\uff3bBFP510 filter \/ 1\u6b21\u30d5\u30a3\u30eb\u30bf\u30fc\u30bb\u30c3\u30c8(481nm short pass)\uff3d \u4ed8\u5c5e\u88c5\u7f6e \uff1aVarirays\uff08\u86cd\u5149\u52b1\u8d77\u7528\u843d\u5c04\u5f0fLED\u5149\u6e90\u30e6\u30cb\u30c3\u30c8\uff09 \u4ed8\u5c5e\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2 \uff1aImageSaver6\uff08\u5236\u5fa1\u7528\u30bd\u30d5\u30c8\uff09 \u3010\u8a2d\u7f6e\u5834\u6240\u3011 \u8a66\u6599\u5206\u6790\u5ba4\uff083\u968e\u3000307\u53f7\u5ba4\uff09 \u3010\u6982\u8981\u3011 \u30fbCCD\u30ab\u30e1\u30e9\u3092-30\u2103\u51b7\u5374\u3059\u308b\u3053\u3068\u3067\u30ce\u30a4\u30ba\u3092\u4f4e\u6e1b \u30fb\u64ae\u5f71\u6642\u306b\u30c0\u30fc\u30af\u30a4\u30e1\u30fc\u30b8\u3092\u6e1b\u7b97\u3057\u3001\u66f4\u306b\u30ce\u30a4\u30ba\u4f4e\u6e1b\u51e6\u7406 \u30fb\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u3088\u304f\u5229\u7528\u3055\u308c\u308b\u6709\u8272\u5206\u5b50\u91cf\u30de\u30fc\u30ab\u30fc\u50cf\u3068\u767a\u5149\u753b\u50cf\u306e\u5408\u6210\u3082\u53ef\u80fd \u3010\u6a5f\u5668\u5229\u7528\u6599\u91d1\u5bfe\u8c61\u3011 \u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044 \u3010\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u5bfe\u8c61\u3011 \u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u304c\u5fc5\u8981\u3067\u3059\uff08\u5404\u7814\u7a76\u5ba41\u3064\u3001\u7814\u7a76\u4ee3\u8868\u8005\u304c\u7533\u8acb\uff09\u3002\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002 \u3010\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u5bfe\u8c61\u3011 \u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u3078\u306e\u767b\u9332\u304c\u5fc5\u8981\u3067\u3059\uff08\u5229\u7528\u8005\u500b\u3005\u304c\u7533\u8acb\uff09\u3002\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u306e\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002 \u3010\u5229\u7528\u4f8b\u3011 \u30fb\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u30ce\u30fc\u30b6\u30f3\u30d6\u30ed\u30c3\u30c8\u306e\u767a\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3001\u86cd\u5149\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0(\u73fe\u5728\u306fGFP\u306e\u307f) \u30fb\u4ee5\u4e0b\u306f\u30aa\u30d7\u30b7\u30e7\u30f3 \u3000\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u86cd\u5149\u67d3\u8272\uff08SYPRO Ruby, Pro-Q Diamond, Oriole\u306a\u3069\uff09\u3001 <a class=\"continue\" href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=6655\">[Continue]<\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2909,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-6655","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/6655","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=6655"}],"version-history":[{"count":15,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/6655\/revisions"}],"predecessor-version":[{"id":23380,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/6655\/revisions\/23380"}],"up":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/2909"}],"wp:attachment":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=6655"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}