{"id":2650,"date":"2011-11-24T14:46:30","date_gmt":"2011-11-24T05:46:30","guid":{"rendered":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=2650"},"modified":"2025-07-28T16:48:36","modified_gmt":"2025-07-28T07:48:36","slug":"%e3%83%aa%e3%82%a2%e3%83%ab%e3%82%bf%e3%82%a4%e3%83%a0pcr%e6%a4%9c%e5%87%ba%e5%99%a8-steponeplus-real-time-pcr-system","status":"publish","type":"page","link":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=2650","title":{"rendered":"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u691c\u51fa\u5668 StepOnePlus Real Time PCR System"},"content":{"rendered":"<div class=\"wc-shortcodes-row wc-shortcodes-item wc-shortcodes-clearfix\">\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-first \">\n<a href=\".\/wp-content\/uploads\/2011\/11\/steponeplus-1.png\" title=\"steponeplus-1\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2011\/11\/steponeplus-1.png\" title=\"steponeplus-1\" width=\"177\" height=\"243\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-7649\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2011\/11\/steponeplus-1.png 236w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2011\/11\/steponeplus-1-219x300.png 219w\" sizes=\"auto, (max-width: 177px) 100vw, 177px\" \/><\/a>\n<\/div>\n<div class=\"wc-shortcodes-column wc-shortcodes-content wc-shortcodes-one-half wc-shortcodes-column-last \">\n\u3000<br \/>\n<a href=\".\/wp-content\/uploads\/2011\/11\/steponeplus_fig01-1.png\" title=\"steponeplus_fig01-1\" target=\"_blank\" rel=\"noopener noreferrer\"><img loading=\"lazy\" decoding=\"async\" src=\".\/wp-content\/uploads\/2011\/11\/steponeplus_fig01-1.png\" title=\"steponeplus_fig01-1\" width=\"230\" height=\"145\" align=\"middle\" class=\"aligncenter size-thumbnail wp-image-7650\" srcset=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2011\/11\/steponeplus_fig01-1.png 920w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2011\/11\/steponeplus_fig01-1-300x190.png 300w, https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/wp-content\/uploads\/2011\/11\/steponeplus_fig01-1-900x570.png 900w\" sizes=\"auto, (max-width: 230px) 100vw, 230px\" \/><\/a>\n<\/div>\n<\/div>\n<p>\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3001\u75c5\u539f\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u3001SNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3001\u30d7\u30e9\u30b9\uff0f\u30de\u30a4\u30ca\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u5b9f\u884c\u53ef\u80fd<\/p>\n<p>\u3010\u4ed5\u69d8\u3011<br \/>\n\u6a5f\u7a2e \uff1aStepOnePlus Real Time PCR System<br \/>\n\u30e1\u30fc\u30ab\u30fc \uff1a<a href=\"http:\/\/www.appliedbiosystems.jp\/website\/jp\/home\/index.jsp\" title=\"Life Technologies\" target=\"_blank\" rel=\"noopener noreferrer\">Life Technologies\uff08Applied Biosystems\uff09<\/a><br \/>\n\u4f7f\u7528\u53ef\u80fd\u86cd\u5149\u8272\u7d20 \uff1aFAM<SUP>TM<\/SUP>\u3001SYBR Green\u3001VIC\u3001TAMRA<SUP>TM<\/SUP>\u3001ROX<SUP>TM<\/SUP><\/p>\n<p>\u3010\u8a2d\u7f6e\u5834\u6240\u3011<br \/>\n\u6a5f\u5668\u5206\u6790\u5ba4\uff08<a title=\"4\u968e\u3000406\u53f7\u5ba4\" href=\".\/wp-content\/uploads\/2025\/02\/406_20240217.png\" target=\"_blank\" rel=\"noopener noreferrer\">4\u968e\u3000406\u53f7\u5ba4<\/a>\uff09<\/p>\n<p>\u3010\u6982\u8981\u3011<br \/>\n\u30fb\u5358\u6ce2\u9577\u52b1\u8d77\u30684\u8272\u5bfe\u5fdc\u306e\u86cd\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u3092\u642d\u8f09<br \/>\n\u30fb\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e0\u306f10\uff5e100\u03bcL\u3067\u6e2c\u5b9a<br \/>\n\u30fb96Well plate\uff08<span style=\"color: #ff0000;\">0.1mL<\/span>\uff09\u3082\u3057\u304f\u306f<span style=\"color: #ff0000;\">0.1mL<\/span> tube\u3092\u4f7f\u7528<\/p>\n<p>\u3010\u6a5f\u5668\u5229\u7528\u6599\u91d1\u5bfe\u8c61\u3011<br \/>\n<a title=\"\u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\" href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/?page_id=22393\" target=\"_blank\" rel=\"noopener noreferrer\">\u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044<\/a><\/p>\n<p>\u3010\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u5bfe\u8c61\u3011<br \/>\n\u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f<span style=\"color: #ff0000;\">\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8<\/span>\u304c\u5fc5\u8981\u3067\u3059\uff08<span style=\"text-decoration: underline;\">\u5404\u7814\u7a76\u5ba41\u3064\u3001\u7814\u7a76\u4ee3\u8868\u8005\u304c\u7533\u8acb<\/span>\uff09\u3002\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u3010\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u5bfe\u8c61\u3011<br \/>\n\u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f<span style=\"color: #0000ff;\">\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0<\/span>\u3078\u306e\u767b\u9332\u304c\u5fc5\u8981\u3067\u3059\uff08<span style=\"text-decoration: underline;\">\u5229\u7528\u8005\u500b\u3005\u304c\u7533\u8acb<\/span>\uff09\u3002\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u306e\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u3010\u6ce8\u610f\u3011<br \/>\n\u30fb8\u9023\u30c1\u30e5\u30fc\u30d6\u306e\u30ad\u30e3\u30c3\u30d7\u306f\u5fc5\u305aFlat type\u3092\u3054\u4f7f\u7528\u4e0b\u3055\u3044\u3002\u6545\u969c\u306e\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u4e0b\u3055\u3044\u3002<br \/>\n\u30fb<span style=\"color: #ff0000;\">plate\u306b\u76f4\u63a5\u30de\u30b8\u30c3\u30af\u3067\u8a18\u5165\u3092\u3057\u306a\u3044\u3067\u4e0b\u3055\u3044<\/span>\u3002\u30de\u30b8\u30c3\u30af\u304c\u6a5f\u5668\u5185\u90e8\u306e\u30ec\u30f3\u30ba\u3084\u30d6\u30ed\u30c3\u30af\u306b\u4ed8\u7740\u3059\u308b\u3068<span style=\"color: #0000ff;\">\u30c7\u30fc\u30bf\u306b\u5f71\u97ff\u3057\u307e\u3059<\/span>\u3002<\/p>\n<p>\u3010\u88dc\u8db3\u3011<br \/>\n\u30fbPPH(Precision Plate Holder)\u306f8\u9023\u30c1\u30e5\u30fc\u30d6\u7528\u306896Well plate\u7528\u304c\u3042\u308a\u307e\u3059\u3002\u3054\u78ba\u8a8d\u306e\u4e0a\u3001\u4f7f\u7528\u3057\u3066\u4e0b\u3055\u3044\u3002<br \/>\n\u30fb96Well plate\u306f\u65bd\u8a2d\u3067\u5206\u8b72\u3057\u3066\u3044\u307e\u3059\u3002\u5206\u8b72\u3092\u5e0c\u671b\u3055\u308c\u308b\u65b9\u306f\u7ba1\u7406\u5ba4\u306b\u304a\u8d8a\u3057\u4e0b\u3055\u3044\u3002<br \/>\n\u30fb\u7d14\u6b63\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u306a\u3044\u5834\u5408\u3001\u89e3\u6790\u306b\u5f71\u97ff\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u6d88\u8017\u54c1\u30ea\u30b9\u30c8\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\u3002<\/p>\n<p>\u3000<a href=\"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/application\/realtime-consumable_ver2015.pdf\" rel=\"noopener noreferrer\" target=\"_blank\">\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0 \u8a66\u85ac\u30fb\u6d88\u8017\u54c1\u30ab\u30bf\u30ed\u30b0 ver.2015<\/a>\u3000\u5b66\u5185\u9650\u5b9a<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u3000 \u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u3001\u75c5\u539f\u907a\u4f1d\u5b50\u306e\u5b9a\u91cf\u3001SNP\u30b8\u30a7\u30ce\u30bf\u30a4\u30d4\u30f3\u30b0\u3001\u30d7\u30e9\u30b9\uff0f\u30de\u30a4\u30ca\u30b9\u30a2\u30c3\u30bb\u30a4\u3092\u5b9f\u884c\u53ef\u80fd \u3010\u4ed5\u69d8\u3011 \u6a5f\u7a2e \uff1aStepOnePlus Real Time PCR System \u30e1\u30fc\u30ab\u30fc \uff1aLife Technologies\uff08Applied Biosystems\uff09 \u4f7f\u7528\u53ef\u80fd\u86cd\u5149\u8272\u7d20 \uff1aFAMTM\u3001SYBR Green\u3001VIC\u3001TAMRATM\u3001ROXTM \u3010\u8a2d\u7f6e\u5834\u6240\u3011 \u6a5f\u5668\u5206\u6790\u5ba4\uff084\u968e\u3000406\u53f7\u5ba4\uff09 \u3010\u6982\u8981\u3011 \u30fb\u5358\u6ce2\u9577\u52b1\u8d77\u30684\u8272\u5bfe\u5fdc\u306e\u86cd\u5149\u30d5\u30a3\u30eb\u30bf\u30fc\u3092\u642d\u8f09 \u30fb\u53cd\u5fdc\u30dc\u30ea\u30e5\u30fc\u30e0\u306f10\uff5e100\u03bcL\u3067\u6e2c\u5b9a \u30fb96Well plate\uff080.1mL\uff09\u3082\u3057\u304f\u306f0.1mL tube\u3092\u4f7f\u7528 \u3010\u6a5f\u5668\u5229\u7528\u6599\u91d1\u5bfe\u8c61\u3011 \u300c\u5229\u7528\u6599\u91d1\u300d\u306f\u3053\u3061\u3089\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044 \u3010\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u5bfe\u8c61\u3011 \u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u304c\u5fc5\u8981\u3067\u3059\uff08\u5404\u7814\u7a76\u5ba41\u3064\u3001\u7814\u7a76\u4ee3\u8868\u8005\u304c\u7533\u8acb\uff09\u3002\u6a5f\u5668\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002 \u3010\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u5bfe\u8c61\u3011 \u30fb\u672c\u6a5f\u306e\u5229\u7528\u306b\u306f\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u3078\u306e\u767b\u9332\u304c\u5fc5\u8981\u3067\u3059\uff08\u5229\u7528\u8005\u500b\u3005\u304c\u7533\u8acb\uff09\u3002\u4e88\u7d04\u30b7\u30b9\u30c6\u30e0\u306e\u30a2\u30ab\u30a6\u30f3\u30c8\u767a\u884c\u306b\u3064\u3044\u3066\u306f\u7ba1\u7406\u5ba4\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u4e0b\u3055\u3044\u3002 \u3010\u6ce8\u610f\u3011 \u30fb8\u9023\u30c1\u30e5\u30fc\u30d6\u306e\u30ad\u30e3\u30c3\u30d7\u306f\u5fc5\u305aFlat type\u3092\u3054\u4f7f\u7528\u4e0b\u3055\u3044\u3002\u6545\u969c\u306e\u539f\u56e0\u3068\u306a\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u4e0b\u3055\u3044\u3002 \u30fbplate\u306b\u76f4\u63a5\u30de\u30b8\u30c3\u30af\u3067\u8a18\u5165\u3092\u3057\u306a\u3044\u3067\u4e0b\u3055\u3044\u3002\u30de\u30b8\u30c3\u30af\u304c\u6a5f\u5668\u5185\u90e8\u306e\u30ec\u30f3\u30ba\u3084\u30d6\u30ed\u30c3\u30af\u306b\u4ed8\u7740\u3059\u308b\u3068\u30c7\u30fc\u30bf\u306b\u5f71\u97ff\u3057\u307e\u3059\u3002 \u3010\u88dc\u8db3\u3011 \u30fbPPH(Precision Plate Holder)\u306f8\u9023\u30c1\u30e5\u30fc\u30d6\u7528\u306896Well plate\u7528\u304c\u3042\u308a\u307e\u3059\u3002\u3054\u78ba\u8a8d\u306e\u4e0a\u3001\u4f7f\u7528\u3057\u3066\u4e0b\u3055\u3044\u3002 \u30fb96Well plate\u306f\u65bd\u8a2d\u3067\u5206\u8b72\u3057\u3066\u3044\u307e\u3059\u3002\u5206\u8b72\u3092\u5e0c\u671b\u3055\u308c\u308b\u65b9\u306f\u7ba1\u7406\u5ba4\u306b\u304a\u8d8a\u3057\u4e0b\u3055\u3044\u3002 \u30fb\u7d14\u6b63\u306e\u6d88\u8017\u54c1\u3092\u4f7f\u7528\u3057\u306a\u3044\u5834\u5408\u3001\u89e3\u6790\u306b\u5f71\u97ff\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4ee5\u4e0b\u306e\u6d88\u8017\u54c1\u30ea\u30b9\u30c8\u3092\u3054\u53c2\u7167\u4e0b\u3055\u3044\u3002 \u3000\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0 \u8a66\u85ac\u30fb\u6d88\u8017\u54c1\u30ab\u30bf\u30ed\u30b0 ver.2015\u3000\u5b66\u5185\u9650\u5b9a<\/p>\n","protected":false},"author":2,"featured_media":0,"parent":2646,"menu_order":0,"comment_status":"open","ping_status":"open","template":"","meta":{"footnotes":""},"class_list":["post-2650","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/2650","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=2650"}],"version-history":[{"count":24,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/2650\/revisions"}],"predecessor-version":[{"id":23370,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/2650\/revisions\/23370"}],"up":[{"embeddable":true,"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=\/wp\/v2\/pages\/2646"}],"wp:attachment":[{"href":"https:\/\/gene4.knit.kagoshima-u.ac.jp\/grc\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=2650"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}